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The premise of Michael Crichton's Jurassic Park and the Steven Spielberg movie that it spawned (along with its sequel, The Lost World) is simple enough. Scientists extract dinosaur DNA remnants lingering in the stomachs of insects entombed in amber for millions of years, reconstitute them into complete copies of dinosaur DNA, and then "grow" dinosaurs inside the lab. It sounds intuitively plausible--if far-fetched--but could it really work? In this fascinating book, Rob DeSalle and David Lindley explain in detail how scientists might attempt this painstaking task and the challenges they would face. In the process, they provide a running tutorial on the techniques of genetic engineering and play spoilsport to the occasional sloppy science of the Crichton and Spielberg works. The result is thoroughly entertaining yet simultaneously enlightening.
Customer Reviews:
The Fantasy and Hope Behind a Would-be Live Dinosaur.......2005-03-29
This book begins with a good overview of our understanding dinosaur biology. There is a description of how the notion of dinosaurs being stupid, lumbering, cold-blooded beasts has given way to the notion of them being at least partly warm-blooded. The discoveries of iridium by the Alvarez team, and how it has revolutionized our understanding of possible dinosaur extinction, is recounted.
The authors freely acknowledge that we know little about DNA. It is frozen in mammoths, but not in dinosaurs. They also acknowledge (p. 17, 42) that the idea of useable dinosaur blood inside an insect trapped in amber is conjecture. If nothing else, the digestive enzymes in an insect's stomach would probably pulverize the DNA long before an even prompt "amberization" could immobilize them. Any dinosaur DNA would almost certainly be broken into fragments, so it would be a Herculean task using overlapping segments to attempt to recreate the dinosaur's complete genome. Moreover, if the DNA was all cleaved in the same position, reassembly would be virtually impossible. Even if reconstructed, it would be challenging to get a dinosaur DNA to work together with, say, within ostrich cell.
However, more modest goals may be attainable in the foreseeable future. For instance, sections of dinosaur DNA may have discernable functions once implanted into the genomes of current organisms. Anything beyond that is farfetched by today's standards of knowledge.
Explanations of the fundamental problems using Dino DNA.......2003-09-05
"Jurassic Park" and "The Lost World" are two movies that involve dinosaurs that have been recreated using advanced genetic engineering techniques. They are exciting and intense as the sight of the dinosaurs makes your heart race. Based on books by Michael Crichton, the engineering strategies used to develop the dinosaurs are explained in a great deal of detail. With all the advancements in genetic engineering, the obvious question to ask is, " Is it now possible to recreate dinosaurs?" The purpose of this book is to answer that question, and the explanations are very well done.
The primary focus is on the many problems of obtaining viable dinosaur DNA, properly sequencing it and getting it to reproduce in a viable manner. These problems are currently overwhelming and the authors explain it in a manner that requires at most the knowledge acquired in high school science classes.
What I liked most about the book is that the authors do not stop after explaining the problems with genetics. Other problems thoroughly discussed deal with difficulties such as the natural immunities that dinosaurs born in their natural habitat would have acquired but would no longer be available, what kind of foods that they would eat and how many dinosaurs could the islands really support. These are questions that would have created additional problems and collectively would have prevented a viable dinosaur ecology from being formed.
Fortunately, movies do not have to be based on solid science to be exciting. In this book you learn the holes in the science which formed the premise of the two movies. Well written and informative, it kept me interested from the first page to the last.
Informative, but tedious and repitious at times.......1999-03-16
Good book, but the authors writing styles are somewhat 'horrible'! Very repititous in some parts. A decent buy...(I lost my copy, and I will be buying another one and reading it again...)
If your a fan of Jurassic Park get this book, you'll love it.......1999-02-28
I have read both Jurassic Park and the Lost world, and if your like me you wish it could be true. In this book two scientist take you on a first person view of what it would be like to be on Jurassic Park and how (if possible) to build a dinosaur. You will learn first hand how close, or far away Steven Spielberg was to the real thing. You will learn a lot of informaion on building a dinosaur like what you would need, were to fing the DNA (not where you would expect, or Spielberg for that matter.) You go to the labs with the scientist and get to make a baby dinosaur. So if you would like to make a dinosaur later in your life read this book!
If your a fan of Jurassic Park get this book, you'll love it.......1999-02-28
I have read both Jurassic Park and the Lost world, and if your like me you wish it could be true. In this book two scientist take you on a first person view of what it would be like to be on Jurassic Park and how (if possible) to build a dinosaur. You will learn first hand how close, or far away Steven Spielberg was to the real thing. You will learn a lot of informaion on building a dinosaur like what you would need, were to fing the DNA (not where you would expect, or Spielberg for that matter.) You go to the labs with the scientist and get to make a baby dinosaur. So if you would like to make a dinosaur later in your life read this book!
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Plant Cell Electroporation And Electrofusion Protocols (Methods in Molecular Biology (Cloth))
JAC A.,ED. NICKOLOFF
Manufacturer: Humana Press
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ASIN: 0896033287 |
Book Description
Leading experts and innovators describe in detail plant cell electroporation and electrofusion techniques for many types of plants. Their protocols cover a diverse set of plant cell types, including different cell types from a single organism (i.e., leaf cells and pollen) and the most important model plants, including maize and tobacco. Each protocol also contains detailed instructions for growth and growth optimization, protoplast generation, and protoplast regeneration for specific plant cell types, along with extensive troubleshooting advice and descriptions of expected results to simplify study design.
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Plant Virology Protocols: FROM VIRUS ISOLATION TO TRANSGENIC RESISTANCE (Methods in Molecular Biology (Cloth))
GARY FOSTER
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ASIN: 0896033856 |
Book Description
A comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus.
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Agrobacterium Protocols (Methods in Molecular Biology (Cloth))
KEVAN,ED. GARTLAND
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Agrobacterium Protocols Volume 1 (Methods in Molecular Biology (Cloth))
ASIN: 0896033023 |
Book Description
Agrobacterium Protocols offers beginning and experienced researchers the most comprehensive collection of step-by-step protocols for the genetic manipulation of plants using Agrobacterium. The topics range from the maintenance of bacterial culture collections to aspects of the metabolism and physiology of transformed tissues and transgenic plants.
Drawing on the work of leading scientists from laboratories around the world, Agrobacterium Protocols provides a wealth of techniques for introducing specific DNA sequences into target plant species and discusses the environmental implications of genetically engineered plants. Its detailed procedures will facilitate rapid transfer of advanced techniques to other laboratories and their exploitation in fundamental and applied plant biology.
Customer Reviews:
Disapointing.......1999-02-27
Although this book claims to have protocols in it. In reality there are very few that have any helpful information. The chapter on binary vectors was a simple review article and did not help in the creation of binary vectors for independent research.
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Agrobacterium Protocols Vol 2 (Methods in Molecular Biology (Cloth))
Kan, Ed. Wang
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Agrobacterium Protocols (Methods in Molecular Biology (Cloth))
ASIN: 1588298434 |
Book Description
A gold standard collection of Agrobacterium-mediated transformation techniques for state-of-the-art plant genetic engineering, functional genomic analysis, and crop improvement. Volume 1 details the most updated techniques available for twenty-six plant species drawn from cereal crops, industrial plants, legume plants, and vegetable plants, and presents various methods for introducing DNA into three major model plant species, Arabidopsis thaliana, Medicago truncatula, and Nicotiana. The authors also outline the basic methods in Agrobacterium manipulation and strategies for vector construction. Volume 2 contains another thirty-three proven techniques for root plants, turf grasses, woody species, tropic plants, nuts and fruits, ornamental plants, and medicinal plants. Additional chapters provide methods for introducing DNA into non-plant species, such as bacteria, fungi, algae, and mammalian cells. The protocols follow the successful Methods in Molecular Biologyâ„¢ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
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Food- Borne Pathogens: Methods and Protocols (Methods in Biotechnology)
Catherine C., Ed. Adley
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ASIN: 158829465X |
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A collection of readily reproducible classic and emerging molecular methods for the laboratory isolation and identification of the pathogens, viruses, and parasites that cause food-borne disease. Among the pathogens covered are specific bacteria, including Salmonella spp, Campylobacter spp., Listeria spp., and Bacillus spp.; viruses, including noroviruses and enteroviruses; and parasites, including Cryptosporidium and seafood nematode worms. The protocols follow the successful Methods in Biotechnologyâ„¢ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
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Genetic Engineering - Principles and Methods (Genetic Engineering: Principles & Methods)
Jane K., Ed. Setlow
Manufacturer: Kluwer Academic Publishers
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ASIN: 0306461846 |
Book Description
Aspects of genetic engineering research emphasized in this volume are applications to plants (crop plants and grass, both important for human needs) and new methodologies, such as Tar cloning, which make it much easier to isolate specific regions from complex genomes. Another subject discussed is linear DNA replication of prokaryotes.
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In vitro Haploid Production in Higher Plants: Volume 1: Fundamental Aspects and Methods (Current Plant Science and Biotechnology in Agriculture)
Manufacturer: Springer
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ASIN: 0792335775 |
Book Description
The 18 chapters making up
In Vitro Haploid Production in Higher Plants are divided into two sections. Section 1 (eight chapters) covers historical and fundamental aspects of haploidy in crop improvement. Section 2 deals with methods of haploid production, including anther culture, micropore culture, ovary culture, pollination with irradiated pollen,
in vitro pollination, and special culture techniques, including polyhaploid production in the Triticeae by sexual hybridization, the influence of ethylene and gelling agents on anther culture, conditional lethal markers, and methods of chromosome doubling.
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Methods for Risk Assessment of Transgenic Plants: IV. Biodiversity and Biotechnology (Methods for Risk Assessment of Transgenic Plants)
Klaus Ed. Ammann
Manufacturer: Springer-Verlag
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ASIN: 3764366575 |
Book Description
This volume is an edited account of an international workshop in Bern about the effects of transgenic plants on biodiversity, a hotly debated topic in the risk assessment research community. Well-known scientists in this field present important new results from their work. Main focus is put on the impact of agricultural biotechnology on ecosystems, social, ethical and legal issues and the impact of biotechnology on conservation. Furthermore, methodological questions, like how to judge biosafety aspects stemming from given research results, are discussed.
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Methods In Plant Molecular Biology And Biotechnology
Bernard, R., Ed. Glick
Manufacturer: CRC Press
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ASIN: 0849351642 |
Book Description
Methods in Plant Molecular Biology and Biotechnology emphasizes a variety of well-tested methods in plant molecular biology and biotechnology. For each detailed and tested protocol presented, a brief overview of the methodology is provided. This overview considers why the protocol is used, what other comparable methods are available, and what limitations can be expected with the protocol. Other chapters in the book present overviews regarding how to approach particular problems and introduce unique methods, such as how to use computer methodology to study isolated genes. The book will be a practical reference for plant physiologists, plant molecular biologists, phytopathologists, and microbiologists.
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Molecular Methods In Plant Pathology
Singh
Manufacturer: CRC
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ASIN: 0873718771 |
Book Description
Molecular Methods in Plant Pathology covers methods in phytopathology at the molecular level, including PCR techniques, electron microscopy, tissue culturing, and the cloning of disease-resistant genes. Phytopathologists, botanists, horticulturists, and anyone working in agriculture will find this a useful reference on biophysical, biochemical, biomolecular, and biotechnological methods.
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