Adhesion Protein Protocols (Methods in Molecular Biology (Cloth))

Book Description

The second edition of Adhesion Protein Protocols combines traditional
techniques with cutting-edge and novel techniques that can be adapted
easily to different molecules and cell types. The topics discussed in
this updated second edition include novel techniques for studying
cell-cell adhesion, neutrophil chemotaxis, in vitro assays used to
study leukocyte migration through monolayers of cultured endothelial
cells, and novel techniques to purify pseudopodia from migratory cells.

This book also discusses the study of cell-matrix interaction, RNA
interference, fluorescence recovery after photobleaching, actin
purification, the application of microarray techniques, and the role of
adhesion proteins in the study of proteomics.

The protocols discussed in this volume are suitable for both novice and
expert scientists, who will gain further insight into the complex and
incompletely understood processes involved in cellular adhesion.

Bioinformatics: Methods and Protocols (Methods in Molecular Biology (Cloth))

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Good to see whats around, Bad for developers
An overview of the software tools for Molecular biology

Bioinformatics: Methods and Protocols (Methods in Molecular Biology (Cloth))
Stephen Misener
Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 0896037320

Book Description

Hands-on users and experts survey the key biological software packages, offering useful tips and an overview of current developments. Among the sequence analysis systems reviewed are GCG, Omiga, MacVector, DNASTAR, PepToolâ„¢, GeneToolâ„¢, and the Staden Package. Molecular biology software includes Genotator and sequence-similarity searching using FASTA, CLUSTAL multiple sequence alignment, and phylogenetic analysis. Web-based resources are examined for primary sequence databases, primary sequence analysis methods, and clinical databases useful in molecular medicine. Numerous illustrative examples to teach the reader how to solve problems are included. Bioinformatics: Methods and Protocols constitutes a gold-standard reference for today's scientists who wish to develop and hone their bioinformatics skills towards the discovery of new biological relationships.

Customer Reviews:

Good to see whats around, Bad for developers.......2001-05-29

Not a bad book for Bioinformatics users who want to see what is around but this kind of information dates very rapidly.

Not a very good book for people interested in developing for bioinformatics (although it may prevent you from reinventing the wheel)

An overview of the software tools for Molecular biology.......2000-03-25

This book gives an overview of the powerful suite of software tools available for various computer applications of molecular biology. The initial chapters talk about many programs(comparisons, Database searching, sequence analysis, Gene Finding, pattern recognition, etc.) and web-based interfaces in addition to the utilities for sequences, databases etc., packed in Wisconsin package apart from short briefs of how to use these tools in a desktop environment. Also this book gives the details of sequence analysis tool called Omiga and its diverse functionalities.

Biological Sequence analysis software like MacVector and Lasergene gets enough attention in this book. Also sequence type-dependent and platform-independen tools like PepTool and GeneTool are also explained in detail.

Two important applications in molecular biology, gene identification and biological pattern detection have received a greater significance here. There is also a chapter about network computing for biologists.

This book lists out a set of free molecular biology software available in different web servers. A lot of relevant information about the journals online, research institutions and organisations and biological information repositories with their web site details and references are also accommodated. There are separate chapters for web-based resources for NCBI(National Center for Biotechnology Information)and EBI(European Bioinformatics Institute) besides a number of computing resources for geneticists.

In contrast to the notion of using mathematical and computational methods for solving different problems in modern biology, there is a chapter on computing with DNA, which talks about using chemical properties of DNA sequences to design a universally programmable molecular computer.

The editors have taken greater efforts in covering almost all vital tools and packages like Staden package, FASTA3 package, CLUSTAL programs, Primer3 on the WWW and Genotator in addition to phylogenetic Analysis using PHYLIP and low cost gel analysis.

On the other hand, the editors could have asked for a couple of more chapters on designing CORBA servers towards true interoperability of diverse biological databases, wrapping of legacy applications , integrating various biological software tools and desiging user-friendly graphical user interfaces using the new entrants like Java and CORBA.

Book Description

The field of clinical proteomics has rapidly evolved during the past few years and is continuously growing as new methodologies and technologies emerge. In Clinical Proteomics, a select group of leading researchers has contributed their state-of-the-art methodologies on protein profiling and identification of disease biomarkers in tissues, microdissected cells and body fluids. Experimental approaches involving the application of two-dimensional electrophoresis, multi-dimensional liquid chromatography, SELDI/MALDI mass spectrometry and protein arrays, as well as the bioinformatic and statistical tools pertinent to the analysis of proteomics data, are thoroughly detailed in these readily reproducible methods and protocols, explained through the step-by-step instructional format of the highly successful Methods in Molecular Biologyâ„¢ series.

Gene Essentiality: Protocols and Bioinformatics (Methods in Molecular Biology (Cloth))

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Gene Essentiality: Protocols and Bioinformatics (Methods in Molecular Biology (Cloth))
Andrei L., Ed. Osterman
Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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Book Description

This book contains a comprehensive collection of experimental and computational strategies and techniques for microbial genome-scale essentiality studies, developed and presented by the leading groups in the field. In addition to wet-lab protocols, the book describes (i) statistical methods essential for planning successful large-scale essentiality screens, as well for data evaluation and analysis; (ii) in-silico prediction of gene essentiality using genome-scale reconstructed metabolic models; and (iii) data integration and comparative analysis in the context of genomic databases. All protocols follow the successful Methods in Molecular Biologyâ„¢ series format, each offering an introduction outlining the principles behind the techniques, step-by-step instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls, and are intended for both novice and expert scientists.

Metabolomics: Methods and Protocols (Methods in Molecular Biology (Cloth))

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Metabolomics: Methods and Protocols (Methods in Molecular Biology (Cloth))
Wolfram Weckwerth
Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 1588295613

Book Description

Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Leading researchers in the field present protocols for the application of complementary analytical methods, such as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies.

Part I of this volume focuses on GC-MS techniques; specifically, attention is given to metabolite profiling in blood plasma, construction and application of mass spectral and retention time index libraries, and metabolomic profiling of natural volatiles. In Part II, emphasis shifts to data integration and data mining techniques, and specific protocols discuss improving pattern recognition and biomarker selection for systems level approaches, and visualization and analysis of molecular and metabolomic data. Parts III, IV, and V examine the coupling of capillary electrophoresis and mass spectrometry, classical reversed phase liquid chromatography-mass spectrometry, and electrochemical detection of metabolites using HPLC separations coupled with coulometric electrode array detectors. In Part VI, protocols for the determination of metabolic flux rates are presented and a theoretical framework for metabolic pathway network analysis is provided. The volume concludes with an examination of cutting-edge NMR protocols.

Plant Bioinformatics: Methods and Protocols (Methods in Molecular Biology (Cloth))

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Plant Bioinformatics: Methods and Protocols (Methods in Molecular Biology (Cloth))
David, Ed. Edwards
Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

Bioinformatics | Biological Sciences | Science | Subjects | Books

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Book Description

Bioinformatics is a rapidly developing field. Data volumes continue to grow and there is an increasing breadth of available data, including sequence, gene expression, protein, metabolite and phenotypic data. Novel databases are being developed for specific species or experiment types. These databases and tools may greatly assist in the application of plant biotechnology, though they require a basic set of skills to identify and utilize the most appropriate resource.

This book aims to assist research scientists in choosing the most applicable database or bioinformatics tools to aid and promote their research in plant biotechnology. Chapters include practical examples and highlight common problems encountered in bioinformatics analysis.

Further chapters are aimed at researchers developing bioinformatics databases and tools, detailing commonly applied database formats and biology-focused scripting languages. These will provide both beginner and experienced bioinformatics researchers with the necessary skills to develop and apply bioinformatics tools for their specialist area of plant research.

Book Description

Provides all new material on urban, industrial, and highway pollution, as well as on management and restoration of streams, lakes, and watershed management techniques.
* Includes revised chapters on agricultural diffuse pollution; control of urban, highway, and industrial diffuse pollution; and wetlands considerations.
* All regulatory data is up to date, with new material provided on judicial law based on significant decisions made in recent years.

Customer Reviews:

Great Book for Dealing With NPS Pollution.......2007-03-26

This is a great book for dealing with Non-Point Source (NPS) pollution. It covers a fairly comprehensive list of topics, while not going into great depth on most. It gives you enough information to get a good start on just about ANY analysis related to NPS pollution and a solid beginning to expand your research from if more in-depth analysis is required. In my opinion, this is a great reference for anyone working in water quality, and a "must-have" for working with NPS.

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Diffuse Pollution

Manufacturer: Elsevier Science Pub Co
ProductGroup: Book
Binding: Paperback

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ASIN: 0080423450

Book Description

Paperback

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Diffuse Pollution '95
Straskraba
Manufacturer: IWA Publishing
ProductGroup: Book
Binding: Paperback

Environmental Science | Earth Sciences | Science | Subjects | Books

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ASIN: 0080428983

Book Description

Paperback

Diffuse Pollution of Water Resources - Principles and Case Studies in the Southern African Region (Balkema--Proceedings and Monographs in Engineering, Water and Earth Sciences)

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Diffuse Pollution of Water Resources - Principles and Case Studies in the Southern African Region (Balkema--Proceedings and Monographs in Engineering, Water and Earth Sciences)

Manufacturer: Taylor & Francis
ProductGroup: Book
Binding: Hardcover

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ASIN: 0415383919

Book Description

Diffuse pollution of water resources has a multi-disciplinary dimension, and its prevention and control is closely related to the development patterns and societal habits of the region. This book bridges the gaps betweem different specialists working in the field and presents an integrated approach for the solution of diffuse pollution problems. It focuses on cases, specific for developing countries and gives emphasis on the need to pursue environmentally sustainable development patterns. Basic principles, definitions and approaches are presented, allowing for the creation of common language and understanding among professionals in the field. Numerous case studies from the region, related mainly to urban sources of diffuse pollution, are included. They could be regarded as typical for any developing country, illustrating different methods to assess and evaluate the extent of diffuse pollution problems. The book serves as a supplementary text for undergraduate and postgraduate students whose studies include a component of water resources and environmental engineering and management, including degree courses in Environmental, Civil and Chemical Engineering, Soil Science, Environmental Sciences and Public Health related sciences. Also, it is also a valuable guide for professionals and managers working in the field of Water Resources and Urban Drainage Management and Environmental protection.

Diffuse/ Non-Point Pollution and Watershed Management (Water Science & Technology)

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Diffuse/ Non-Point Pollution and Watershed Management (Water Science & Technology)
Burkart
Manufacturer: IWA Publishing
ProductGroup: Book
Binding: Paperback

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Water Quality International '94 Part 7: Groundwater Contamination, Environmental Restoration and Diffuse Source Pollution

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Water Quality International '94 Part 7: Groundwater Contamination, Environmental Restoration and Diffuse Source Pollution

Manufacturer: Elsevier Science Pub Co
ProductGroup: Book
Binding: Paperback

Environmental Science | Earth Sciences | Science | Subjects | Books

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Book Description

Paperback

Integrated Management of Water Quality: Role of Agriculture Pollution Sources (Water Science and Technology)

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Integrated Management of Water Quality: Role of Agriculture Pollution Sources (Water Science and Technology)
Bendoricchio
Manufacturer: IWA Publishing
ProductGroup: Book
Binding: Paperback

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ASIN: 0080436331

Book Description

Paperback

Development of an integrated model for assessing the impact of diffuse and point source pollution on coastal waters [An article from: Environmental Modelling and Software]

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Development of an integrated model for assessing the impact of diffuse and point source pollution on coastal waters [An article from: Environmental Modelling and Software]
D. Yuan , B. Lin , R.A. Falconer , and J. Tao
Manufacturer: Elsevier
ProductGroup: Book
Binding: Digital

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ASIN: B000PDT4EK

Book Description

This digital document is a journal article from Environmental Modelling and Software, published by Elsevier in 2007. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
Pollution loads from land sources and their impact on the receiving waters can be predicted by using land-use and surface water quality models, respectively. In this paper details are given of the development of an integrated modelling system for managing water quality in coastal basins. The system includes a model linking the pollution input loads to land-use and a model for simulating surface flow and chemical and biological processes. The land-use model consists of two main components: a database and a model base. It uses a GIS system, namely ArcGIS, as the model platform. The database, built in the Geo-database format, includes six data sets containing information on social, economical, bathymetrical and hydrological aspects of a study area. The model base includes a series of transfer functions that link the pollution loads to key sources, including those from: industrial, domestic and agricultural inputs, and fish and animal farming. The water quality model is a numerical model which solves the governing equations representing the physical, chemical and biological processes in coastal waters. The modelling system was set up for Bohai Bay in China, with the land-use model being used to provide nutrient loadings from individual inputs and the water quality model used to predict the impact of these inputs on the receiving waters. Model simulations were also undertaken to predict the water quality conditions for various development and management scenarios.

Diffuse and point pollution impacts on the pathogen indicator organism level in the Geum River, Korea [An article from: Science of the Total Environment, The]

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Diffuse and point pollution impacts on the pathogen indicator organism level in the Geum River, Korea [An article from: Science of the Total Environment, The]
G. Kim , E. Choi , and D. Lee
Manufacturer: Elsevier
ProductGroup: Book
Binding: Digital

Elsevier | By Publisher | e-Docs | Formats | Books
ASIN: B000RR5N2Q

Book Description

This digital document is a journal article from Science of the Total Environment, The, published by Elsevier in . The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
The pathogens originating from diffuse pollution have raised much concern recently. In many countries, pathogen levels are monitored in surface water by measuring the pathogen indicator organism level, which indicates the concentration of pathogen associated microorganisms to determine contamination. Among indicator organisms, total coliform, fecal coliform, and Escherichia coli were selected for study, and their concentration as well as their flow rate were monitored at monitoring stations from October, 2001 to April, 2003. Monitoring stations include six sampling stations in the Geum River, two small watersheds used for forestry and agricultural land, one large wastewater treatment plant, one separate sewer overflow site, and one separate sewer overflow site in the Geum River basin. The coliform concentration of the combined sewer overflow was the highest, followed by the runoff from agricultural land use, the separate sewer overflow, and the runoff from forestry land use. The Pearson correlation coefficient for flow rate against total coliform concentration was 0.71 and was significant at 0.01 level, while the Pearson coefficient for other water quality constituents showed weak correlation (-0.36 to +0.37) against flow rate. Coliform concentration showed higher correlation against suspended solid concentration or flow rate during storm flow condition than during low flow condition. Two different relationship lines could explain the relationships between the flow rate and coliform loadings. Load duration curve technique was presented to assess the relative contributions of diffuse and point source pollution to the pathogen level at monitoring sites in the Geum River.

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Diffuse Pollution 6
Verstappen
Manufacturer: IWA Publishing
ProductGroup: Book
Binding: Paperback

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