PCR Methods in Foods (Food Microbiology and Food Safety)

Book Description

This book is a primer for students, faculty, and other professionals interested in molecular biology and its integration into food safety, by introducing the reader to diagnostic PCR-based technologies used in detection of pathogens in foods.

This book will enable its reader to:

1. Understand the principles behind PCR including real-time
2. Know the basics involved in the design, optimization, and implementation of PCR in food microbiology lab setting
3. Interpret results
4. Know limitations and strengths of PCR
5. Understand the basic principles behind microarrays and its potential applications in food microbiology.

Figures, charts, and tables help illustrate concepts and provide the reader with an important starting point in bringing molecular diagnostics into the food microbiology lab.

Development and assessment of a rapid method to detect Escherichia coli O26, O111 and O157 in retail minced beef [An article from: International Journal of Hygiene and Environmental Health]

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Development and assessment of a rapid method to detect Escherichia coli O26, O111 and O157 in retail minced beef [An article from: International Journal of Hygiene and Environmental Health]
M. Murphy , A. Carroll , C. Walsh , P. Whyte , and O'Mahon
Manufacturer: Elsevier
ProductGroup: Book
Binding: Digital

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ASIN: B000PDT4L8

Book Description

This digital document is a journal article from International Journal of Hygiene and Environmental Health, published by Elsevier in 2007. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
A molecular-based detection method was developed to detect Escherichia coli O26, O111 and O157 in minced (ground) beef samples. This method consists of an initial overnight enrichment in modified tryptone soya broth (mTSB) and novobiocin prior to DNA extraction and subsequent serogrouping using a triplex PCR. This method has a low limit of detection and results are available within 24 hours of receipt of samples. Once optimized, this rapid method was utilized to determine the prevalence of these E. coli serogroups in six hundred minced beef samples all of which were previously examined by immunomagnetic separation (IMS) and selective plating for E. coli O26 and O111. Using IMS, two E. coli O26 isolates were detected. No E. coli O111 were recovered. The multiplex PCR technique described here did not detect E. coli O111 nor O157 in any of the samples, however six minced beef samples were positive for E. coli O26 using our method, only two of these were previously detected by IMS and culture. Application of molecular methods are useful to support culture-based approaches thereby further contributing to risk reduction along the food chain.

Rapid Cycle Real Time PCR - Methods and Applications: Microbiology and Food Analysis

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Rapid Cycle Real Time PCR - Methods and Applications: Microbiology and Food Analysis

Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

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Real Time PCR (BIOS Advanced Methods)

ASIN: 3540418814

Book Description

Rapid Cycle Real-Time PCR is a powerful analytical tool with broad application for the basic and applied life sciences. Compared with conventional PCR technology, Rapid Cycle Real-Time PCR is faster, has greater specificity, and is more easily adaptable for a variety of diagnostic tests, including qualitative, quantitative and mutation detection assays. This book provides general overviews of this technology for use in the clinical microbiology laboratory as well as specific diagnostic protocols for the detection of viral, bacterial and fungal pathogens and genetically modified organisms in human specimens and foodstuffs. All of these protocols have been developed, verified, and validated by experts in the field and should be of great interest for clinical microbiologists, pathologists, laboratory technologists as well as practicing physicians.

Book Description

After nearly 60 years, with descriptions of more than 270 species and almost 200 illustrations, Textbook of Dendrology continues to remain a top resource for taxonomic and silvicultural information on North American trees. In this new edition, material throughout the text has been updated and expanded to provide current information on tree sizes, damaging diseases and insect pest, economic uses, and silvics. Because of growing concern for the environment, it is even more necessary for students to know and understand the importance of conservation. Many of these issues are incorporated throughout the book.

Systematics and the Origin of Species from the Viewpoint of a Zoologist

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Still and always a key reference

Systematics and the Origin of Species from the Viewpoint of a Zoologist
Ernst Mayr
Manufacturer: Harvard University Press
ProductGroup: Book
Binding: Paperback

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ASIN: 0674862503

Book Description

Ernst Mayr is perhaps the most distinguished biologist of the twentieth century, and Systematics and the Origin of Species may be one of his greatest and most influential books. This classic study, first published in 1942, helped to revolutionize evolutionary biology by offering a new approach to taxonomic principles and correlating the ideas and findings of modern systematics with those of other life science disciplines. This book is one of the foundational documents of the "Evolutionary Synthesis." It is the book in which Mayr pioneered his new concept of species based chiefly on such biological factors as interbreeding and reproductive isolation, taking into account ecology, geography, and life history. In his new introduction for this edition, Mayr reflects on the place of this enduring work in the subsequent history of his field.

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Still and always a key reference.......2000-03-31

This book belongs on every serious biologist's bookshelf -when it isn't on your desk, in your easy chair, in the clutches of your students, etc. Not as "easy a read" as Mayr's more "popular" books like the wonderful ONE LONG ARGUMENT, this was and is a definitive statement on key elements of the evolutionary synthesis. Get it & read it!

Green Plants: Their Origin and Diversity

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Great upper-level book, but not for an intro course
Basic botany text.

Green Plants: Their Origin and Diversity
Peter R. Bell , and Alan R. Hemsley
Manufacturer: Cambridge University Press
ProductGroup: Book
Binding: Paperback

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ASIN: 0521646731

Book Description

The central theme of Green Plants is the astonishing diversity of forms found in the plant kingdom. The book is arranged according to generally accepted classification schemes, beginning with prokaryotic and eukaryotic algae and moving through mosses, liverworts, fern allies, ferns and gymnosperms to flowering plants. Copiously illustrated throughout, it provides a concise account of all algae and land plants, with information on topics from cellular structure to life cycles and reproduction. The authors include newly emerging information on features of plants known only as fossils. This new edition has been completely updated to reflect current views on the origin of the major plant groups.

Customer Reviews:

Great upper-level book, but not for an intro course.......2002-06-23

I was needing a book for an introductory botany course, covering plant evolution and structure. This book is too advanced for a course like that, but would be excellent for a 300 or 400 level course after students have the basics of evolution, taxonomy, and plant structures down. As a grad student, this is a text I'd love to have as a resource and will use as a reference text in my course...but it's too dry and the language is at too high a level for beginners. I am recommending this text to a professor of a botany class I was TA'ing for on Comparative Plant Anatomy, a 300 level course. So...if you want to be a botanist, grab it. If you want to teach plant anatomy and origins after an introductory botany course, this is perfect (best I've seen for the price, too!). If you want to teach an AP Botany course to high school students that only have a high-school biology background with no botany experience....not a good choice except as a strong reference. Book explains well, has excellent black-and-white diagrams, follows a thorough and logical pattern of evolution, but simply uses terminology too advanced for a lower-level course. They have trouble with the concepts of transcription and translation, much less page 103, "The passage of materials into the sporophyte is entirely through the apoplast since in all archeoniates the boundary between the two phases lacks plasmodesmata." Clear to me....but not to a beginner. I can't stress how good this book is for an advanced course or a serious botanist/taxonomist/plant geneticist/etc. GREAT upper-level/professional text!!!!!

Basic botany text........2001-04-30

This is a short book on plant diversity. There are lots of B/W drawings and diagrams. The book can be used in botany classes or for amateurs who want to learn more about plants.

Systematics and the Origin of Species (Columbia Biological Series, No. 13.)

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Systematics and the Origin of Species (Columbia Biological Series, No. 13.)
Ernst Mayr
Manufacturer: Columbia University Press
ProductGroup: Book
Binding: Paperback

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The Variety of Life: A Survey and a Celebration of All the Creatures that Have Ever Lived

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My best biology book
You will never look at life on earth the same way again
The Fantastic Panorama of Life
A magnificent reference text for biologists
In fiction this would be an epic!

The Variety of Life: A Survey and a Celebration of All the Creatures that Have Ever Lived
Colin Tudge
Manufacturer: Oxford University Press, USA
ProductGroup: Book
Binding: Hardcover

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ASIN: 0198503113

Amazon.com

It takes a brave writer to tackle the truly Herculean task of describing The Variety of Life with the astronomical numbers of organisms living today, let alone all those that have fallen by the wayside over the billions of years of life on Earth. No one is quite sure how many living species there are, but it is estimated to be somewhere between 10 million and 100 million. Fortunately, since the days of the great Swedish naturalist Linnaeus, around 250 years ago, life has been grouped and classified into hierarchical schemes. As a result, it is possible to encompass this enormous variety of life by describing the relatively few groups into which it can be clustered. And, since the mid-19th century and the Darwin-Wallace theory of evolution by natural selection, classification has taken on an extra, evolutionary dimension.

Colin Tudge, a well-known British science writer, has training in whole animal biology and a self-proclaimed love for the natural-historical foray among our fellow creatures. The first part of this big book (all of 90 pages) deals with the thorny problems of what Tudge rightly calls the craft and science of classification. Since the 1950s, the word cladistics has terrorized many traditional naturalists and biologists. But it is here to stay, and Tudge provides a very welcome guide that will be invaluable to both lay people and students.

The bulk of the text, nearly 500 pages, forms part II and includes the descriptions of the main groups, from the most primitive (alpha proteobacteria) prokaryotes to Eupatorium, a large genus of 1,800 or so species of plant. In between these two groups, at either end of the biological spectrum, lie all the more familiar bugs and beasts, including ourselves. Inevitably, given so many millions of organisms, difficult choices have to be made. Some groups are only dealt with at phylum level (for example, brachiopods), while others are detailed down to family level (for example, primates). Some extinct groups (not surprisingly, the dinosaurs) get a look, but not many overall. The short epilogue concerns conservation and is followed by a useful reference list of sources and an index. Altogether, the 600-odd pages are enlivened with a large number of excellent black-and-white drawings of individual organisms and diagrams illustrating evolutionary relationships. For all natural historians and anyone interested in biology, the The Variety of Life is a must. --Douglas Palmer, Amazon.co.uk

Book Description

The Variety of Life can be read at many levels. Not least it is an extraordinary inventory - an illustrated summary of all the Earthly creatures that have ever lived. Whatever living thing you come across, from E coli to an oak tree or an elephant, The Variety of Life will show you what kind of creature it is, and how it relates to all the others. Yet there are far too many creatures to present merely as a catalogue. The list of species already described is vast enough - nearly two million - but there could in reality be as many as 30 million different animals, plants, fungi and protists - and perhaps another 400 million different bacteria and archaes. In the 4000 million years or so since life first began on Earth, there could have been several thousand billion different species. The only way to keep track of so many is to classify - placing similar creatures into categories, which nest within larger categories, and so on. As the centuries have passed, so it has become clear that the different groups are far more diverse than had ever been appreciated. Thus Linneus in the 18th century placed all living things in just two kingdoms, Animals and Plants. By the 1950s this had become five kingdoms - with fungi, protists, and bacteria hived off into their own, separate groups. But leading biologists today acknowledge three vastly different domains, each divided into many kingdoms - so that animals and plants, spectacular though they are, are just a fragment of the whole. The Variety of Life explains the means by which systematists have attempted such a mammoth classification of so many various creatures - which in turn leads us into some of the most intriguing and knottiest areas of modern biology: evolutionary theory, molecular genetics, and the history of biological thought. Finally, however, The Variety of Life can simply be seen as a celebration. We should all share Miranda's pleasure in Shakespeare's Tempest - ' How many goodly creatures are there here!' - and feel, as she did, what a privilege it is to share this planet with such wonders. Their fate is in our hands; and first, we must begin to appreciate them.

Customer Reviews:

My best biology book.......2006-06-22

It is hard to imagine why anyone could give Tudge's book less than 5 stars. With what must you compare the work, to justify less than 5? Tudge has a friendly, graceful style, the book is full of most fascinating information and I have not seen any even remotely comparable biology text. And any reader of Tudge tempted to give credence to one of the numerous primitive myhths about origins, such as those in the Bible, must be moved to wonder how even the most diligent Divine Design Department could have produced such a vast number of different species in 6 days - e g 300,000 sorts of beetles, 120,000 species of butterflies and moths, literally millions of sorts of bacteria and so forth. As scientists so often argue, and Tudge amply demonstrates, the facts we now know are more interesting and marvellous than the ancient myths.

You will never look at life on earth the same way again.......2004-03-02

Professor Tudge has done all of us a great service with this terrific book. He lays out a clear way for generalists to get a basic understanding on the way life on this planet is related at present and into the past to our best understanding of life's origins.

He explains a variety of classification systems (and some specialists might disagree with his characterizations - but that is a smallish point to those of us who aren't specialists) and provides wonderful illustrations that give us a broad sweep of how the branches flow together in the past. He explains the current limits of our understanding. And he has a wonderful treatment of the Domains as currently understood - Bacteria, Archae, and Eucarya. Obviously, most of the book is on Eucarya because that is most interesting to us humans, but the bulk of life on earth is bacteria and that is kind of interesting to understand.

This book really updates my understanding of what I was taught in 7th grade biology too many years ago. I think every bright high school student ought to read it as well as anyone who wants to understand the amazing range of life now living and that has lived on this earth. You won't look at your life here the same way ever again.

The Fantastic Panorama of Life.......2003-07-21

Colin Tudge has produced a remarkable book that captures the complexities of the Earth's biota. Probably already somewhat out of date (phylogenic studies are producing new results at a fantastic rate) this book is still a necessary reference for biologists everywhere. The old two-kingdom concept, which gave way to a five-kingdom concept, is now a multi-kingdom concept. At the very least we should have six kingdoms- Animalia, Fungi, Plantae, Protoctista, Archaebacteria and Eubacteria. The exact final number is yet to be decided. However, it can be easily argued that the Protoctista and the Bacteria could be broken into even more kingdoms and indeed several authors now talk of at least three domains, containing procaryote (bacterial) and eucaryote kingdoms.

All of this is primarily a result of studies on DNA and other chemicals of life. This research has especially shown the bacterial and "single-celled" organism world to be much more complex than anyone ever thought. From slime molds to cyanobacteria and oak trees to humans, the variation on life on this planet is what fascinates biologists. Tudge's book is a very good review of this extreme diversity and gives us a very good reason to avoid destroying it! Read this book if you are interested in the diversity of life on Earth.

A magnificent reference text for biologists.......2003-05-20

This book pulls together an enormous amount of information and makes it digestible to the average undergraduate - no small feat. It's scope is magnificent, as is its treatment of fundamental concepts of evolution. Although I think there are some problems with the sections on extinct birds and cetaceans (based on new discoveries), Tudge does as well as anybody could in defining outgroups and sister taxa, always erring on the conservative side. I think the most novel and thought-provoking portion of the text concerns the number of kingdoms we might now wish to recognize - I discuss Tudge's reasoning for this with my biology undergraduates and it never fails to make an impression. A splendid accomplishment, and I'm waiting eagerly for a second edition, and a third, and so on. Well done Dr. Tudge, and sincere thanks.

In fiction this would be an epic!.......2003-02-19

An imposing book by a major science writer, Tudge rightly subtitles this work "a celebration." Although at first glance the book seems overwhelming, Tudge has broken down his feast of life into easily consumed portions. After an excellent overview of the history of classifying life, he allows the reader to choose among the many types of animals and plants. One can jump to insects, birds, fish or reptiles for more detailed evolutionary accounts and modern examples. Unable to resist, i skimmed over a few more esoteric examples to settle down to Primates and Hominids. This section provides a superb overview of current knowledge, distinguishing clearly what is known and what is supposed. This was familiar territory but delving in the other sections proved equally rewarding. However, this also suggests a warning that the book is not a "cover-to-cover" exercise.

Tudge opens with the problem facing many new students of biological sciences - how to deal with the immensity of information confronting them. There are, he notes, over two million species described already. No-one disputes the number is far below the actual total life contains - but what is the realistic total? Estimates range as high as 100 million - an almost inconceivable figure. He accepts the more likely total as around thirty million, recognizing that such numbers remain out of human ken. From this, he builds his case that classification systems are necessary. What's required is a classification method that anyone can grasp. He finds the solution in the idea proposed by German entomologist Willi Hennig - cladistics. This system arranges life by characteristics, avoiding confusing generalities and the arcane mysteries of genetics. As Tudge argues, cladistics has become fourth phase of classification systems, and the one likely to endure.

The "technical" sections of the book, covering the multitude of life forms each open with a descriptive essay followed by a "tree" of relationship among various species. This structure makes the book an excellent reference work and will keep it valuable for many years. The illustrations are designed to impart general information, not scientific detail. Neither are they simplistic as the supporting comment provides pointers to consider when viewing them. Tudge groups the text and graphics nicely, allowing visual and text comparison without constant page flipping.

As with any author confronting the immense cargo of information available in biology, Tudge was forced into a selective process in creating a bibliography. It's not an enviable task. The list appears sparse, a heavily pruned tree arranged by chapters. He indicates his preferred references, but only by using his sources will you discover whether more bountiful reading is listed in them. This lack in no way impairs the worth of this effort, however. There are countless book lists available. Anyone with an interest in life will treasure this volume.

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Superb technical book for angiographers

Arterial Variations in Man: Classification and Frequency
H. Lippert
Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

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ASIN: 038700341X

Customer Reviews:

Superb technical book for angiographers.......1998-11-11

This is a superb book for angiographers (Medical Doctors / Radiologists who take X-Ray pictures of the blood vessels of patients. It is superbly referenced and authoratative. It documents the variations of the blood vessels of humans. Instead of the vague statements of other books that this or that variation "occurrs, this book documents all the variations in easy to read graphical format AND gives percentages and references the source of that data! Most unusual in the text book business.

Book Description

Such is the pressure on teaching time in schools and universities that students are taught less and less of the diversity that is life on this planet. Most students, and indeed most professional biologists that these students become, know far more of cell function than of biodiversity. This text is profusely illustrated, quick reference guide to all types of living organism from the single-celled prokaryotes and eurkaryotes to the multicellular fungi, plants and animals. All surviving phyla and their component classes are characterised and described, as are their life-styles, ecology, relationships and within-group diversity (with orders displayed in list form). Overall, the book's aim is to provide biologists and others with a clear, concise picture of the nature of all groups of organisms with which they may be unfamiliar.

The Elements of Inductive Logic: Designed Mainly for the Use of Students in the Universities

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The Elements of Inductive Logic: Designed Mainly for the Use of Students in the Universities
Thomas Fowler
Manufacturer: Adamant Media Corporation
ProductGroup: Book
Binding: Paperback
ASIN: 1402186940
Release Date: 2003-06-05

Book Description

This Elibron Classics book is a facsimile reprint of a 1870 edition by the Clarendon Press, Oxford.

Evolution, Variation, and Classification of Palms (Memoirs of the New York Botanical Garden) (Memoirs of the New York Botanical Garden)

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Evolution, Variation, and Classification of Palms (Memoirs of the New York Botanical Garden) (Memoirs of the New York Botanical Garden)

Manufacturer: New York Botanical Garden
ProductGroup: Book
Binding: Hardcover

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ASIN: 0893274267

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