Plant Virology Protocols: From Virus Isolation to Transgenic Resistance (Methods in Molecular Biology)

Book Description

A comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus.

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Molecular Biology of Plant Viruses

Molecular Biology of Plant Viruses

Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

Biochemistry | Biological Sciences | Science | Subjects | Books

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Book Description

Two decades ago, recombinant DNA technology or genetic engineering ushered in a new era in the study of plant viruses. The major breakthrough came in the eighties, primarily due to the development of new methods for RNA reverse transcription and cDNA cloning, along with restriction enzyme mapping and rapid nucleotide sequencing. An information explosion in various molecular aspects of plant viruses was caused by these studies. Current research efforts investigate the study of viral genomes, genetic maps, genes and gene expression, gene products, and genetic basis of virus functions and biological properties.
Molecular Biology of Plant Viruses analyzes, collates and reviews such published information. Additionally, it demonstrates the mechanisms of genetic variability; brings out the molecular basis of virus transport in plants and of virus transmission by vectors and of disease symptomatology; and discusses molecular biology of viroids and transgenic plants. It also treats the recently discovered genetic phenomenon of gene silencing and the gene-for-gene interactions between the hosts and plant viruses.
Molecular Biology of Plant Viruses is an excellent reference, providing insight into the exciting research developments made in the field.

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Molecular Biology of Plant Viruses.......2000-04-01

Although there are several well known books dealing with human viruses and their biology, this recently published monograph edited by Dr. Mandahar fills the need for an up to date review of plant viruses and their molecular biology. Thirteen chapters written by experts in their respective fields cover gene expression, genetic variability, viral transport and transmission, gene to gene interaction and gene silencing of RNA and DNA containing plant viruses. Addition of references and a subject index makes it easy to review the pertinent information in this 320-page monograph. Most of the articles are written by experts in their respective fields and cover the historical literature as well as the recent developments. This monograph should be an excellent source of information for researchers interested in plant viruses and their molecular biology. Dr. Mandahar has earlier published three other multiauthor books dealing with plant viruses.

Plant Infectious Agents: Viruses, Viroids, Virusoids, and Satellites (Current Communications in Cell and Molecular Biology)

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Plant Infectious Agents: Viruses, Viroids, Virusoids, and Satellites (Current Communications in Cell and Molecular Biology)
Stephen H. Howell , Russel L. Malmberg , Hugh D. Robertson , and Zaitlin
Manufacturer: Cold Spring Harbor Laboratory Pr
ProductGroup: Book
Binding: Paperback

General | Gardening & Horticulture | Home & Garden | Subjects | Books

Microbiology | Basic Science | Medicine | Subjects | Books
ASIN: 087969159X

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Plant Viruses As Molecular Pathogens

Manufacturer: Haworth Press
ProductGroup: Book
Binding: Hardcover

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Viral Genes and Plant Pathogenesis
Thomas P. Pirone
Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

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Microbiology | Basic Science | Medicine | Subjects | Books

Epitope Mapping (Molecular Biology Intelligence Unit Series)

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Epitope Mapping (Molecular Biology Intelligence Unit Series)
Andy Soltys
Manufacturer: Chapman & Hall
ProductGroup: Book
Binding: Hardcover

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Molecular Biology of Plant Viruses
J. W. Davies , T. M. A. Wilson , and Simon N. Covey
Manufacturer: Chapman & Hall
ProductGroup: Book
Binding: Paperback

General | Evolution | Science | Subjects | Books

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ASIN: 0412314606

Book Description

Recent understanding of the involvement of carbohydrate molecules and conjugates in many vital biological processes and, consequently, the appreciation of their tremendous therapeutic potential has stimulated the development of new methods for the synthesis of this class of compounds. The main efforts in the field of synthetic carbohydrate chemistry have been focused on the development of new glycosylation methodologies and convergent strategies for oligosaccharide synthesis, in which the number of synthetic and purification steps is reduced. As a result, many efficient approaches have been developed both on the solid phase and in solution. This book brings together leading specialists working in the emerging areas of chemistry and biology on specific topics in the area of carbohydrates. As a result, the book will be of great interest to scientists solving carbohydrate puzzles in many diverse areas of research.

Biocatalysis in Polymer Science (Acs Symposium Series)

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Biocatalysis in Polymer Science (Acs Symposium Series)

Manufacturer: An American Chemical Society Publication
ProductGroup: Book
Binding: Hardcover

General & Reference | Chemistry | Science | Subjects | Books

Catalysis | Chemistry | Science | Subjects | Books

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ASIN: 0841237816

Book Description

Biocatalysis in Polymer Science provides current information on biocatalysis, which is the use of enzymes or living organisms to carry out chemical reactions. All of the authors, including the editors, are experts and practitioners of polymer biocatalysis. Topics covered in this book include synthetic polymers, natural polymers, oligomers, and monomers. These topics amply demonstrate the versatility of this technology. Organic chemists, polymer chemists, biochemists, and graduate students will find this book a useful experience.

Alkene Metathesis in Organic Synthesis (Topics in Organometallic Chemistry)

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Alkene Metathesis in Organic Synthesis (Topics in Organometallic Chemistry)

Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

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Book Description

The tremendous progress in olefin metathesis achieved during the last decade can hardly be overestimated. Due to the development of a new generation of well defined and high-performance organometallic catalysts, this reaction is rapidly evolving into one of the most versatile tools for advanced organic chemistry, natural product synthesis, fine chemical production and polymer sciences. Written by some of the leading experts in this field, this monograph intends to familiarize the reader with the most exciting developments and frontiers in this flourishing field of chemistry research.

Catalysis in Polymer Synthesis (Acs Symposium Series)

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Catalysis in Polymer Synthesis (Acs Symposium Series)

Manufacturer: An American Chemical Society Publication
ProductGroup: Book
Binding: Hardcover

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Polymers & Macromolecules | Chemistry | Science | Subjects | Books

Catalysis | Chemistry | Science | Subjects | Books

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Book Description

Examines the role of catalysis in applied polymer developments, focusing on metal catalysis of olefins, epoxides, oxetanes, cyclic phosphazenes, and cyclic silanes. Provides discussion of metal catalyzed coordination polymerization of olefins and diolefins and ring opening coordination polymerization of epoxides and oxetanes. Covers recent developments on anionic and cationic polymerization. Places particular emphasis on early and recent developments on propylene polymerization as related to isotactic and syndiotactic propylene. Provides a review of the work of E.J. Vandenberg, recipient of the 1991 ACS Award in Applied Polymer Science, in olefin, epoxide, and oxetane polymerization.

Novel Metathesis Chemistry: Well-Defined Initiator Systems for Specialty Chemical Synthesis, Tailored Polymers and Advanced Material Applications (NATO ... II: Mathematics, Physics and Chemistry)

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Novel Metathesis Chemistry: Well-Defined Initiator Systems for Specialty Chemical Synthesis, Tailored Polymers and Advanced Material Applications (NATO ... II: Mathematics, Physics and Chemistry)

Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

General & Reference | Chemistry | Science | Subjects | Books

Inorganic | Chemistry | Science | Subjects | Books

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ASIN: 1402015704

Book Description

A comprehensive discussion of the theory, practice and application of catalytically active transition metal species and their application in the production of specialist polymeric materials. The material is presented in a progressive manner, suitable for non-experts and those seeking an introduction to the field. The bibliographies supplied are complete and up to date, making the book an indispensable guide to the primary literature for the more theoretical background to the topics discussed.
After a comprehensive discussion of initiating systems for speciality chemical synthesis the book goes on to deal with a wide range of topics in materials science, including: alkenamers, polyacetylenes, industrial applications and liquid rocket engine fuels.

Organometallic Catalysts and Olefin Polymerization: Catalysts for a New Millennium

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Organometallic Catalysts and Olefin Polymerization: Catalysts for a New Millennium

Manufacturer: Springer-Verlag Telos
ProductGroup: Book
Binding: Hardcover

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Catalysis | Chemistry | Science | Subjects | Books

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ASIN: 3540414029

Oxygenates by Homologation or CO Hydrogenation with Metal Complexes (Catalysis by Metal Complexes)

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Oxygenates by Homologation or CO Hydrogenation with Metal Complexes (Catalysis by Metal Complexes)

Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

General & Reference | Chemistry | Science | Subjects | Books

Inorganic | Chemistry | Science | Subjects | Books

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ASIN: 0792326288

Book Description

The homogeneous catalytic hydrogenation of CO and the carbonylation and homologation of alcohols and their derivatives emerged nearly 40 years ago, in the U.S.A. and Germany. Since that time, the two topics have generally been reviewed separately, with no concern for their common aspects, in terms of both the chemical transformations involved, nor for the catalytic aspects, until Japanese workers, during the National C ₁ Chemistry Project, which ran from 1980 to 1987, unified both the scientific and technological approaches in the field. The literature from 1940--1980 has been well reviewed, and so the time is now ripe for a thorough, careful review of work accomplished since then.
Under the editorial inspiration of Professor Braca, Oxygenates by Homologation or CO Hydrogenation with Metal Complexes presents an authoritative reconsideration and review of research on oxygenate production by CO hydrogenation, and the homologation of alcohols, aldehydes, ethers and esters in the presence of transition metal complexes. The three chapters of the book, each presenting a masterly overview of its topic, are entitled: Monoalcohols, Glycols and their Ethers and Esters by CO Hydrogenation; Alcohols and Derivatives by Homologation with SynGas; and Hydrocarbonylation of Aldehydes and their Derivatives.
The chapters are presented in a common format, presenting the performances of the catalytic systems of different metals, followed by a general discussion of the reaction mechanism along the successive reaction steps, from the activation of the substrates up to their chemical modification and the restoration of the catalysts. Attention is also paid to the problems of stability and deactivation of the catalytic systems and to the difficulties of the industrial development of the reactions.

(2nd text)
This volume presents an overview of the current status of research into homogeneous phase catalytic carbon monoxide hydrogenation and the carbonylation and homologation of alcohols and their derivatives, with emphasis on the progress which has been made since 1982.
The concents are divided into three major chapters. Chapter 1 deals with monoalcohols, glycols and their ethers and esters by carbon monoxide hydrogenation. Chapter 2 discusses alcohols and their derivatives by homologation with syngas. Chapter 3 considers the hydrocarbonylation of aldehydes and their derivatives.
The organization of the material in each of these chapters follows a common scheme. The performance of catalytic systems of different metals is presented and then reaction mechanisms are discussed for successive reaction steps, from the activation of substrates to their chemical modifications and the restoration of catalysts. Attention is also paid to problems of stability and deactivation of catalytic systems. The difficulties associated with the industrial development of the various reactions is also considered.
For research organic chemists in academia and industry whose work involves catalysis.

Polymer Synthesis/Polymer Catalysis (Advances in Polymer Science)

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Polymer Synthesis/Polymer Catalysis (Advances in Polymer Science)
B. Ameduri , M. Arndt , B. Boutevin , T. Endo , Ph Gramain , W. Kaminsky , E. Ruckenstein , and Y. Yagci
Manufacturer: Springer-Verlag Telos
ProductGroup: Book
Binding: Hardcover

General & Reference | Chemistry | Science | Subjects | Books

Polymers & Macromolecules | Chemistry | Science | Subjects | Books

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Polymeric Materials in Organic Synthesis and Catalysis

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Polymeric Materials in Organic Synthesis and Catalysis

Manufacturer: Wiley-VCH
ProductGroup: Book
Binding: Hardcover

General & Reference | Chemistry | Science | Subjects | Books

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Book Description

This is the first book to describe the synthesis and characterization of the materials used in polymer-supported synthesis. The authors cover not only the classical polymers and their use in homogeneous, heterogeneous and micellar catalysis, but also such new developments as "enzyme-labile linkers", illustrating how to simplify the purification process and avoid waste. The result is a wealth of useful information -- for beginners and experts alike - in one handy reference, removing the need for difficult and time-consuming research among the literature.

Volume 1, Metallocene-Based Polyolefins: Preparation, Properties, and Technology

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Volume 1, Metallocene-Based Polyolefins: Preparation, Properties, and Technology

Manufacturer: Wiley
ProductGroup: Book
Binding: Hardcover

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Catalysis | Chemistry | Science | Subjects | Books

Book Description

The manufacture of polyolefins by metallocene catalysts represents a revolution in the polymer industry. The first, patent for a metallocene catalyst was filed in 1980 but it has been the last five years that have seen a dramatic increase in the volume of research into metallocenes and the maturing of metallocene technology. With contributions from leading experts from the US, Canada, Italy, Scandinavia, Germany and Japan, Metallocene-based Polyolefins gives comprehensive coverage of all areas of metallocene technology: catalyst structure, comonomer incorporation, polymerization mechanisms and conditions, reactor configurations, special properties, rheological and processing behaviour, comparison with conventional polyolefins and fields of application. An essential book for plastics engineers, polymer chemists, physicists, materials scientists and all those working in the plastics manufacturing and processing industries.

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