Cell Biology: A Laboratory Handbook, Four-Volume Set

Book Description

This four-volume laboratory manual contains comprehensive state-of-the-art protocols essential for research in the life sciences. Techniques are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls. The important steps and results are beautifully illustrated for further ease of use. This collection enables researchers at all stages of their careers to embark on basic biological problems using a variety of technologies and model systems.

This thoroughly updated third edition contains 165 new articles in classical as well as rapidly emerging technologies.

Topics covered include:
* Cell and Tissue Culture: Associated Techniques, Viruses, Antibodies, Immunocytochemistry (Volume 1)
* Organelle and Cellular Structures, Assays (Volume 2)
* Imaging Techniques, Electron Microscopy, Scanning Probe and Scanning Electron Microscopy, Microdissection, Tissue Arrays, Cytogenetics and In Situ Hybridization, Genomics and Transgenic Knockouts and Knock-down Methods (Volume 3)
* Transfer of Macromolecules, Expression Systems, Gene Expression Profiling (Volume 4)

* Indispensable bench companion for every life science laboratory
* Provides the latest information on the plethora of technologies needed to tackle complex biological problems
* Includes numerous illustrations, some in full color, supporting steps and results

Customer Reviews:

Greatest value of this work is its comprehensiveness.......2001-02-03

Cell biology techniques have been continuously upgraded since the middle of this century, and future developments seem assured. In the 1990s, alone, new developments have led to improved separations capability, detection instrumentation, and new formulations of serum-free media. Any research institution with faculty and students engaged in cell biology will want to have the most current laboratory guidelines available, and this four-volume, peer-reviewed collection of protocols answers that need. The second edition has benefited from suggestions from practicing scientists, filling in gaps from the first edition.

Perhaps the greatest value of this work is its comprehensiveness. Laboratories and libraries can have detailed guidelines for cell culture, separation, staining, growth, apoptosis, preparation of component parts, assays, antibody production, genetic engineering, microscopy, electrophoresis, and more, all in one source. Need to know how to produce transgenic mice? It's here. How to grow viruses? Prepare nuclei? Stain mitochondria? Use fluorecence microscopy? All here.

Book Description

Cell culture techniques are invaluable to the modern researcher but difficult to carry out successfully. As part of the series of Handbooks in Practical Animal Cell Biology, this volume offers a concise practical guide to the basic essentials of the technique. Researchers new to cell culture will find a clear explanation of the essential equipment of a tissue culture facility, including tissue culture media and sera. It describes methods for growing suspension and adhesion cultures, including how to store cells and prepare primary cultures from cells. For those already culturing cells, the handbook will act as a handy reference to the basic techniques. The essence of the book is to deal with the generalities of cell culture and to give a grasp of the basic concepts before involvement in more specialized work in the field. Ideal for anyone moving into tissue cell culture techniques or looking for a concise reference book.

Customer Reviews:

From Book to Bench-Cell Culturing Made Easy.......2000-05-16

Whether a first year college student or a seasoned researcher, General Techniques of Cell Culture is a must. GTCC simplifies the complicated world of cell culturing without insulting. The authors, Maureen A. Harrison and Ian F. Rae, make no assumptions about their readers, and take the task of explaining large amounts of scientific information in an organized and easy to read format. This handbook goes beyond general lab techniques and provides not only the "how-to", but also gives the developing lab the where at, as it rounds out each section with a directory of services and researchable references. By providing time honored techniques, General Techniques of Cell Culture also takes some of the trial and error out of propagating healthy cells in the lab. This alone should make the transition from book to bench less tedius and more productive.

Haematopoietic and Lymphoid Cell Culture (Handbooks in Practical Animal Cell Biology)

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Haematopoietic and Lymphoid Cell Culture (Handbooks in Practical Animal Cell Biology)

Manufacturer: Cambridge University Press
ProductGroup: Book
Binding: Paperback

Anatomy | Biological Sciences | Science | Subjects | Books

Experiments & Projects | Experiments, Instruments & Measurement | Science | Subjects | Books

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ASIN: 0521629691

Book Description

This invaluable reference handbook describes the fundamental principles and procedures underlying the successful isolation of viable, functionally intact hematopoietic and lymphoid cells, and their maintenance as primary cultures. The text provides technical information on the signals and mediators required for the differentiation and growth of these cells, and is designed for laboratory investigators with limited practical experience in cell culture. Chapters discuss dendritic cells, T and B lymphocytes, monocytes and macrophages, NK and LAK cells, mast cells and basophils, hematopoietic differentiation of embryonal stem cells, and the culturing of murine thymic explants. Each chapter has been written by experts who have practical experience of the techniques discussed to provide tips for avoiding common pitfalls, and sharing insight into the fundamental questions in cell biology and immunology addressed using each cell culture model.

Epithelial Cell Culture (Handbooks in Practical Animal Cell Biology)

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Epithelial Cell Culture (Handbooks in Practical Animal Cell Biology)

Manufacturer: Cambridge University Press
ProductGroup: Book
Binding: Paperback

Experiments & Projects | Experiments, Instruments & Measurement | Science | Subjects | Books

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ASIN: 052155991X

Book Description

Epithelial Cell Culture contains chapters by experts on epithelial cells derived from the airway, intestine, pancreas, kidney and bladder, genital ducts, mammary glands, skin glands and appendages, and keratinocytes.

Bacterial Toxins: Tools in Cell Biology and Pharmacology (Laboratory Companion)

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Bacterial Toxins: Tools in Cell Biology and Pharmacology (Laboratory Companion)

Manufacturer: Chapman & Hall
ProductGroup: Book
Binding: Hardcover

Biochemistry | Biological Sciences | Science | Subjects | Books

Bacteriology | Agricultural Sciences | Science | Subjects | Books

Toxicology | Pharmacology | Medicine | Subjects | Books

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Book Description

This is a survey of well characterized and recently discovered bacterial protein toxins. Leading investigators of the respective toxins review the various molecular mechanisms of action, ranging from toxin-induced ADP-ribosylation up to membrane perforation by pore-forming toxins. Thy also describe the consequences on host physiology before focusing on potential applications as cell biological and pharmacological tools for research and medical applications. Detailed descriptions of the methodology include the engineering and use of modified and chimeric toxins for better performance.
A solid introduction to toxin structure and functions, as well as a valuable source of methodology for researchers in molecular biology, pharmacology and experimental medicine.

Endocrine Cell Culture (Handbooks in Practical Animal Cell Biology)

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Endocrine Cell Culture (Handbooks in Practical Animal Cell Biology)

Manufacturer: Cambridge University Press
ProductGroup: Book
Binding: Paperback

Anatomy | Biological Sciences | Science | Subjects | Books

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Endocrinology & Metabolism | Internal Medicine | Medicine | Subjects | Books

Book Description

This handy reference describes the fundamental principles and procedures underlying the successful isolation of viable, functionally intact cells from mammalian endocrine tissues, and their maintenance as primary cultures. The cell types selected for coverage illustrate the diversity of endocrine tissues from which cells have been isolated, and the range of procedures that have been devised to ensure the optimal survival and behavior of each cell type under study. The book places particular emphasis on the provision of detailed protocols describing, step by step, the manipulations necessary to establish differentiated and responsive cultures. The chapters have been prepared by authors who have direct practical experience with the cell types covered, therefore providing the reader with first-hand accounts on the background to each procedure, the avoidance of potential problems and pitfalls, and the fundamental questions in endocrinology that may be addressed using each cell culture model.

Endothelial Cell Culture (Handbooks in Practical Animal Cell Biology)

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Endothelial Cell Culture (Handbooks in Practical Animal Cell Biology)

Manufacturer: Cambridge University Press
ProductGroup: Book
Binding: Paperback

Experiments & Projects | Experiments, Instruments & Measurement | Science | Subjects | Books

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ASIN: 0521559901

Book Description

Endothelial Cell Culture contains chapters by experts on endothelial cells derived from the lung, bone marrow, brain, mammary glands, skin, adipose tissue, female reproductive system, and synovium.

Handbook of Industrial Cell Culture: Mammalian, Microbial, and Plant Cells

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Handbook of Industrial Cell Culture: Mammalian, Microbial, and Plant Cells

Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

Biotechnology | Biological Sciences | Science | Subjects | Books

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Biotechnology | Special Topics | Medicine | Subjects | Books

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ASIN: 1588290328

Book Description

A diverse team of researchers, technologists, and engineers describe, in simple and practical language, the major current and evolving technologies for improving the biocatalytic capabilities of mammalian, microbial, and plant cells. The authors present state-of-the-art techniques, proven methods, and strategies for industrial screening, cultivation, and scale-up of these cells, and describe their biotech and industrial uses. Special emphasis is given to the solving critical issues encountered during the discovery of new drugs, process development, and the manufacture of new and existing compounds. Other topics include recombinant protein expression, bioinformatics, high throughput screening, analytical tools in biotechnology, DNA shuffling, and genomics discovery.

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Marrow Stromal Cell Culture

Manufacturer: Cambridge University Press
ProductGroup: Book
Binding: Paperback

Anatomy | Biological Sciences | Science | Subjects | Books

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ASIN: 0521589789

Book Description

The first book of its kind on marrow stromal cells, and written by leading experts in the field, this concise handbook aims to provide all that is needed to those involved in this relatively young area of research. The book provides a historical perspective and a summary of present knowledge of the marrow stromal lineages that are currently best understood. The contributors provide detailed protocols for the isolation, culture, and characterization of marrow stromal cell types from human and other animal species. The breadth and depth of the subject matter included makes the book equally suitable for new researchers and the more experienced investigator in this rapidly expanding field.

Cell Biology: A Laboratory Handbook (cell Biology)

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Cell Biology: A Laboratory Handbook (cell Biology)
Julio E. Celis
Manufacturer: Academic Press
ProductGroup: Book
Binding: Paperback
ASIN: B000OHM8PO

Book Description

This book is an engineering guide for design of slopes and stabilisation works in rocks and residual soils. It is tailored to the needs of practising geotechnical engineers and engineering geologists. Engineering and engineering geology students will find it quite useful and a practical course guide. It can be used as textbook in courses on landslides and slope stabilisation. The purpose of this book is to present a concise documentation on how to design slopes and how to select a slope stabilisation method. The authors are scholars and professional engineers with many years of international experience in slope stabilization works in South and Central America and the Far East.

Handbook of Slope Stabilization Engineering

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Handbook of Slope Stabilization Engineering
Jose A. Ortigao
Manufacturer: NY
ProductGroup: Book
Binding: Hardcover
ASIN: B000MUFFQC

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