Basic Cell Culture (Practical Approach Series)

Book Description

The use of animal, including human, cell culture has expanded enormously during the last 25 years, with new applications appearing all the time. The aim of this book is to guide the newcomer progressively through all those areas which nowadays are basic to the performance of cell culture. The book should also prove useful to the experienced worker when entering a new field, setting up a new laboratory, or as a source of reference on basic techniques. Revised and updated chapters cover topics such as equipping a cell culture laboratory, sterilization, culture media, basic culture technique, cell cloning, the isolation of new cell lines, contamination procedures, and good laboratory practice. There is a brand new chapter on the microscopy of living cells, and an appendix of online resources for cell biology.

Basic Cell Culture Protocols (Methods in Molecular Biology)

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Basic Cell Culture Protocols (Methods in Molecular Biology)

Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 1588292843

Book Description

In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side-population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis).

Sickle Cell Disease: Basic Principles and Clinical Practice

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Sickle Cell Disease: Basic Principles and Clinical Practice
Stephen H. Embury , and Robert P. Hebbel
Manufacturer: Lippincott Williams & Wilkins
ProductGroup: Book
Binding: Hardcover

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Book Description

Written by 80 of the world's foremost basic scientists and clinicians, this volume is the first comprehensive reference on sickle cell disease. Because this disease has diverse manifestations and involves many medical specialties, the contributors were chosen for their expertise in specific areas. Their discussions cover virtually every aspect of the disease--its molecular and cellular biology, pathophysiology, diagnosis, organ-specific complications, and clinical management. Coverage begins with recent laboratory findings on sickle hemoglobin and sickle red cell pathology. Subsequent sections examine the pathophysiology and natural history of sickle cell disease and describe diagnostic methods, including prenatal diagnosis and neonatal screening. A major section focuses on strategies for management of the organ-specific complications and psychosocial aspects of the disease. Coverage of therapy includes antibiotics, transfusion, surgery, pain control, nursing concerns, and future prospects for cure of the disease by gene therapy, bone marrow transplantation, and drugs that induce fetal hemoglobin synthesis.

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Basic Cell Culture Protocols (Methods in Molecular Biology)

Manufacturer: Humana Press
ProductGroup: Book
Binding: Paperback

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ASIN: 0896033848

Book Description

Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, 2nd ed. offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. Like the first edition, this book should be in every cell culture laboratory and be of use to all who use cell cultures in research.

Cell Chemistry and Physiology: Part IV (Principles of Medical Biology)

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Good way to cell chemistry

Cell Chemistry and Physiology: Part IV (Principles of Medical Biology)

Manufacturer: Elsevier Science
ProductGroup: Book
Binding: Hardcover

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Book Description

This volume is intended to complete the Cell Chemistry and physiology module. It is about how the traditional boundaries of cell chemistry and physiology are being erased by molecular biology. We do not think it necessary to elaborate on this theme, particularly since the body of core knowledge found in this volume brings us a stage closer to answering the question, "what makes cell biology into a new discipline?"
The first part of the volume deals with the chemistry of actin and myosin and is followed by chapters on cell motility, ATP synthesis in muscle, and contraction in smooth and skeletal muscle. Here the reader is immediately made aware of the contributions molecular biology is making to our understanding of the molecular mechanisms underlying muscle contraction. It is perhaps enough to point out that Huxley's concept of the cross-bridge cycle and generation of force can now be explained in molecular terms. Topics such as muscle fatigue and muscle disorders, as well as malignant hyperthermia are bound to arouse active learning in the student and set the stage for problem-based learning.
Most medical students look askance at thermobiology. We think this is a mistake; hence, we have included a section dealing with this subject. This brings us to the chapter on the heat shock response, which at the very outset makes clear that many stressors besides heat are known to result in heat shock gene expression. Many of the heat shock proteins occur in unstressed cells and some of them behave as chaperones. These proteins also reach high levels in a wide range of diseases including neurodegenerative disorders. Whether certain diseases are the result of mutations in the heat shock genes is not yet known. As will be appreciated, much of the work done in this field involved the use of cultured cells. Animal cells in culture are the subject of the last chapter.

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Good way to cell chemistry.......2003-01-28

This book has some good title and context about ion transport across the cell membrane. Understanding of ions transport is very essentail way to study of cell membrane functions and its responses to stimuli. It is very good for any biological and medical students that have well known information about ionic events in and out of the cell. Some students don't suppose how ions act and transport across the cytoplasmic membranes. This book make better idea for ions function and ionic behavior in cells. Chapers of the book have well arrangement and good figures. I recommend it for study.

Glial Cell Development: Basic Principles and Clinical Relevance

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Glial Cell Development: Basic Principles and Clinical Relevance

Manufacturer: Oxford University Press, USA
ProductGroup: Book
Binding: Hardcover

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Book Description

This is a new edition in the Cellular and Molecular Neurobiology series. The vast majority of cells in the nervous system are glial cells. During development, these cells provide growth factors that stimulate the proliferation, migration and survival of neurones and their precursors, and promote and guide axonal growth. In the mature nervous system, glial cells provide insulating myelin sheathe around axons and provide metabolic and structural support for neurones. Glial cells also have a major influence on the local response to injury of the central nerve tract and the peripheral nervous system, either promoting, or inhibiting, axonal regrowth and recovery of lost function. This book provides a comprehensive, state-of-the-art overview of research into the development, function and malfunction of glial cells. It offers a compelling insight into how basic research throws light onto diseases and disorders and points the way towards treatments. Teams of internationally renowned experts, all active in research, have contributed chapters.

Animal Cell Culture and Technology: The Basics (Basics (Oxford, England))

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Animal Cell Culture and Technology: The Basics (Basics (Oxford, England))
Michael J. Butler
Manufacturer: Oxford University Press, USA
ProductGroup: Book
Binding: Paperback

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ASIN: 0199634165

Book Description

Animal cell culture is an important laboratory technique in the biological and medical sciences. Indeed it has become an essential tool for the study of most biochemical and physiological processes, The use of large-scale animal cell culture has become increasingly important to the commercial
production of specific compounds for the pharmaceutical industry. This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable
introduction to animal cell culture for undergraduates, graduates, and experienced researchers.

Adhesion Molecules and Cell Signaling: Biology and Clinical Applications (Topics in Molecular Medicine, Vol 1)

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Adhesion Molecules and Cell Signaling: Biology and Clinical Applications (Topics in Molecular Medicine, Vol 1)
Reinbard Lorenz
Manufacturer: Raven Pr
ProductGroup: Book
Binding: Paperback

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Book Description

This is the first volume of a new Raven Press series, Topics in Molecular Medicine. The series brings together leading basic and clinical scientists to explore the clinical applications of new insights into the molecular mechanisms of physiologic and pathophysiologic processes. Volume 1 focuses on the role of adhesion molecules in regulating physiologic and pathophysiologic cell-cell interactions in the circulation, the lymphatic system, and extravascular space. The first two sections examine the structure and regulation of the main classes of adhesion molecules: integrins, selectins, and intercellular adhesion molecules (ICAMs). Subsequent sections describe the role of these molecules in postangioplasty reactions, ischemia-reperfusion injury, atherogenesis, other vascular disorders, metastasis, and immunologic and inflammatory responses such as asthma and transplant rejection.

Advances in Hematopoietic Stem Cell Transplantation and Molecular Therapy: Proceedings of the Symposium "Hematopoietic Steam Cell Transplantation and Gene Therapy (Recent Results in Cancer Research)

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Advances in Hematopoietic Stem Cell Transplantation and Molecular Therapy: Proceedings of the Symposium "Hematopoietic Steam Cell Transplantation and Gene Therapy (Recent Results in Cancer Research)

Manufacturer: Springer-Verlag Telos
ProductGroup: Book
Binding: Hardcover

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ASIN: 3540626263

Book Description

Blood stem cell transplantation has become an established therapeutic option in the treatment of malignant diseases. At the same time molecular therapeutic approaches (i.e. gene therapy) are promising alternatives to the classical treatment of cancer. The proceedings give an overview of the biology of hematopoietic progenitor cells, peripheral stem cell transplantation in patients with hematological malignancies, recent developments in molecular diagnosis and gene therapeutic concepts, and show that successful future developments require cooperative research connecting basic science with clinical application.

Animal Cell Technology: Basic & Applied Aspects: Volume 6

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Animal Cell Technology: Basic & Applied Aspects: Volume 6

Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

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Book Description

Animal cell technology is a growing discipline of cell biology which aims to understand the structure, function and behaviour of differentiated animal cells, and especially the development of such abilities as are useful for industrial purposes. These developments range from clonal expansion of differentiated cells with useful abilities, to optimization of cell culture on industrial scale and modulation of the cells' abilities to produce drugs and monoclonal antibodies. The sixth volume in this series gives a complete review of today's state of the art in Japan, a country where this field is especially well advanced. It will be of interest to cell biologists, biochemists, molecular biologists, immunologists and other disciplines related to animal cell culture, working in the academic environment as well as in (biotechnology or pharmaceutical) industry.

Book Description

The present monograph presents, for the first time, a unified and comprehensive introduction to some of the basic transport properties of porous media, such as electrical and hydraulic conductivity, air permeability and diffusion. The treatment is based on critical path analysis and the scaling of transport properties which are individually described as functions of saturation.

At the same time, the book supplies a tutorial on percolation theory for hydrologists, providing them with the tools for solving actual problems. In turn, a separate chapter serves to introduces physicists to some of the language and complications of groundwater hydrology necessary for succesful modelling.

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