The Yeast Two-Hybrid System (Advances in Molecular Biology)

Book Description

Biologists have already identified thousands of new gene sequences, and genome sequencing efforts are speeding up the discovery process even further. With this explosion of sequence information comes the need to understand how genes work in concert in order to fulfill the cells functions. The yeast two-hybrid system--used to identify protein-protein interactions--is one of the most powerful and versatile methods for characterizing a protein's function. It has become an essential tool for both academic researchers and those in biotechnology and pharmaceutical companies. This volume presents work by pioneers in the field and is the first publication devoted solely to the yeast two-hybrid system. It includes detailed protocols, practical advice on troubleshooting, and suggestions for future development. In addition, it explains how to construct an activation domain hybrid library, how to identify mutations that disrupt an interaction, and how to use the system in mammalian cells. Many of the contributors have developed new applications and variations of the technique. Chapter topics include characterizing hormone/receptor complexes, identifying peptide ligands, analyzing interactions mediated by protein modifications, and dissecting the cell cycle and other complex genetic networks. The Yeast Two-Hybrid System is the single complete resource for scientists interested in this powerful research method.

E. coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology)

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E. coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology)

Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 1588291510

Book Description

A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.

Advances in Plasmid Molecular Biology (Advances in Plasmid Molecular Biology)

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Advances in Plasmid Molecular Biology (Advances in Plasmid Molecular Biology)

Manufacturer: JAI Press
ProductGroup: Book
Binding: Hardcover

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Bacteria, Plasmids, and Phages: An Introduction to Molecular Biology

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Bacteria, Plasmids, and Phages: An Introduction to Molecular Biology
Edmund Lin
Manufacturer: Harvard University Press
ProductGroup: Book
Binding: Paperback

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Bacterial Conjugation

Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

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ASIN: 0306443767

Book Description

This timely volume reviews the molecular mechanisms and regulation aspects of conjugation in a variety of bacterial systems. An international group of contributors examine the movement of plasmids and conjugative transposons across species and kingdom barriers, and discuss the significance of the transfer phenomenon from clinical, environmental, and industrial perspectives.

Bacterial plasmids (An Addison-Wesley module in biology)

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Bacterial plasmids (An Addison-Wesley module in biology)
Edward A Adelberg
Manufacturer: Addison-Wesley Pub. Co
ProductGroup: Book
Binding: Paperback

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ASIN: 0201110741

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I WANNA GOOD DESCRIPn ABOUT PIJ101;PSC101
A very nice book on plasmids.

The Biology of Plasmids
David K. Summers
Manufacturer: Blackwell Publishing Limited
ProductGroup: Book
Binding: Paperback

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ASIN: 063203436X

Book Description

Plasmids are closed, circular pieces of DNA which are able to self replicate and are carried by many bacteria. They provide unique functions for the bacteria, e.g. by allowing them to sexually replicate and to pass on genetic material between each other. Plasmids are also responsible for the genetic factors that give resistance to antibiotics, and provide the enzymes needed to break down poorly metabolised food resources. The author has provided an up to date treatment of the structure, function and application of plasmids suitable for undergraduates and medical students. An original teaching perspective is employed in that plasmids are examined as living organisms with either a symbiotic or parasitic mode of survival. This gives a very important frame-work for understanding the structure and function of plasmids in an evolutionary context.

Customer Reviews:

I WANNA GOOD DESCRIPn ABOUT PIJ101;PSC101.......1999-04-14

I SHOULD HAVEW A BRIEF EXPLANATION WITH SITE MAPS FOR ALL THE PLASMIDS

A very nice book on plasmids........1997-07-10

The Biology of Plasmids" is not only a very good introduction to an interesting field, but also summarizes information that can be very hard to find anywhere else. It is very well written and has a clear logic structure, showing strong intuition

Extrachromosomal Elements in Lower Eukaryotes (Basic Life Sciences)

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Extrachromosomal Elements in Lower Eukaryotes (Basic Life Sciences)

Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

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From Replication Initiation to Condensation and Partition of Chromosome and Plasmid in Escherichia Coli

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From Replication Initiation to Condensation and Partition of Chromosome and Plasmid in Escherichia Coli
Tao Weitao
Manufacturer: Uppsala Universitet
ProductGroup: Book
Binding: Paperback

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Microbial Linear Plasmids (Microbiology Monographs)

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Microbial Linear Plasmids (Microbiology Monographs)

Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

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Book Description

Linear plasmids of microbes represent a heterogenous group of extrachromosomal genetic elements initially assumed to be rare and peculiar. However, we now know that they are fairly frequently occurring plasmids in bacterial and eukaryotic species. Viral strategies to avoid shortening of the linear molecules during replication imply a common ancestry. Linear plasmids may be beneficial, neutral or detrimental for the respective host; functions include production of protein toxins, specific catabolic capabilities, antibiotic resistance, pathogenicity factors, and senescence induction.

Microbial Linear Plasmids constitutes the first attempt to comprehensively assemble current knowledge of different types of such elements, highlight recent developments in the field, and challenge the distinction between viruses and linear plasmids.

Book Description

Methods of X-ray and Neutron Scattering in Polymer Science presents the basic theories underlying x-ray and neutron scattering--two of the most powerful tools for characterizing materials--and also covers the various techniques that have been developed for their application to the study of polymers. While there is a great deal of similarity between the x-ray and neutron scattering methods, the two were developed in different time periods by different groups of scientists, and as a result very distinct terminologies evolved to explain the same phenomena. In this unique text the two are presented together from the very beginning, with a consistent set of symbols and terminologies, so that students can become equally familiar with both from the outset. Also, to help students gain a unified view of diffraction, the distinction between wide-angle diffraction and small-angle scattering is postponed until late in the text. Methods of X-ray and Neutron Scattering in Polymer Science emphasizes basic concepts rather than details of specific techniques and derives relationships from first premises wherever possible. Beginning with coverage of the basic properties of x-rays and neutrons and their scattering from matter, it goes on to discuss methods of studying specific types of samples or properties. Topics covered include single-component crystalline and amorphous polymers; the small-angle scattering technique; binary, single-phase systems such as polymer blend and polymer solution; the technique of reflectivity measurement; and polymer dynamics by means of inelastic neutron scattering. A perfect introductory textbook for graduate and advanced undergraduate students in polymer science,Methods of X-ray and Neutron Scattering in Polymer Science also serves as a helpful self-study tool for polymer scientists seeking an introduction to scattering techniques. Further reading lists at the end of each chapter encourage readers to explore more advanced topics on their own.

Customer Reviews:

Useful guide on scattering for Polymer Scientists .......2006-06-07

Ryoung-Joon Roe presents a handy reference and introduction to x-ray and neutron scattering, where emphasis is placed on using a terminology that helps the reader learn about both the techniques simultaenously. Scattering has served as one of the most important characterization tools for polymer community. This book outlines the basic mathematics and experimental details required to understand the structure and properties as revealed by these scattering methods in: crystalline and amorphous polymers, polymer solutions and blends, polymer dynamics and block copolymers. While emphasis is placed on demonstrating how all scattering shares similar theories and philosophy, the choice of several examples and applications of either techniques is used to remark on aspects peculiar to either X-ray studies or Neutron Scattering.

Since most serious students of polymer science are familiar with light scattering, the book would appeal them as perfect guide to familiarize themselves with limits and use of neutron and X-ray scattering. A more detailed discussion on Polymers and Neutron Scattering is found in the classic text by Julia S. Higgins and Henry C. Beno^it, while for Light Scatttering texts by Pecora & Berne and by Wyn Brown are essential references. Compared to those classic references, Roe's text will appear as more accessible to people seeking introduction to scattering methods. By the same token, it contains only the essence, the flavor of aspects of polymer behavior, say crystallization, surface studies or dynamics, and one will need to delve into the other texts if he seeks exhaustive discussion.

Radiation Effects on Polymers (Acs Symposium Series)

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Radiation Effects on Polymers (Acs Symposium Series)

Manufacturer: Oxford University Press, USA
ProductGroup: Book
Binding: Hardcover

General & Reference | Chemistry | Science | Subjects | Books

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Book Description

Here is the most extensive resource on polymer radiation effects to be available in more than a decade. This new volume reviews the fundamental chemistry and physics of polymer-radiation interaction and examines recent progress in most major areas of the field. Its 38 chapters, written by leading experts from around the world, cover: fundamentals of polymer radiation chemistry; technological applications of radiation to polymers (including radiation processing; radiation curing; sterilization; cross-linking, polymerization, grafting, X-ray resists, and others); and degradation of stabilization of irradiated polymers (including nuclear plants, scintillation detectors for particle physics, and others).

Catalysis by Polymer-Immobilized Metal Complexes

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Catalysis by Polymer-Immobilized Metal Complexes
Anatoly D. Pomogailo
Manufacturer: CRC
ProductGroup: Book
Binding: Hardcover

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Book Description

Deals with a new and promising field developed during the last two decades on the boundary between homogeneous and heterogeneous catalysis. This book presents general information on catalysis for a wide range of organic reactions, e.g., hydrogenation and oxidation reactions, and polymerization transformations. Special attention is paid to electro- and photochemical stimulation of catalytic processes in the presence of immobilized metal complexes. Other topics covered are the quantitative data on the comparison of catalyses by mobile and immobilized metal complexes; main factors affecting the activity of these catalytic systems and methods of optimizing their control; and specific problems of catalysis by fixed complexes (e.g., ligand exchange and electron transfer in metal polymer systems, macromolecular effects and polyfunctional catalysis).

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Catalysis in Precision Polymerization

Manufacturer: Wiley
ProductGroup: Book
Binding: Hardcover

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ASIN: 047195327X

Book Description

This book provides a comprehensive survey of the entire field of catalysis in polymer synthesis covering the majority of essential aspects from basic organic, organometallic and bio-chemistry to industrial production. Particular emphasis is placed on "precision polymerization," where the catalyst plays a key role in the fabrication of innovative polymers. Precision polymerization enables the production of polymers with precisely controlled structures, molecular weight, distribution and stereochemistry, free from defects.

Current Trends in Polymer Photochemistry

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very interesting and enlightening

Current Trends in Polymer Photochemistry
Norman Allen
Manufacturer: Prentice Hall PTR
ProductGroup: Book
Binding: Textbook Binding

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Book Description

The degradation and stabilisation of polymeric materials is of crucial interest to any chemist working in this field. This book offers up-to-date information on light-related characteristics of polymers, and represents a compilation of specialised topics from prestigious international authorities in polymer photochemistry.

Customer Reviews:

very interesting and enlightening.......1999-06-18

it is like going through a lot of sumarised inf

Dynamic Light Scattering: The Method and Some Applications (Monographs on the Physics and Chemistry of Materials, 49)

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Dynamic Light Scattering: The Method and Some Applications (Monographs on the Physics and Chemistry of Materials, 49)

Manufacturer: Oxford University Press, USA
ProductGroup: Book
Binding: Hardcover

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Book Description

Dynamic light scattering is a new method for investigating macromolecular systems. The importance of the technique lies in its non-invasive character. It can be employed on extremely small fluid volumes, the instrumentation is relatively inexpensive and allows the rapid determination of
diffusion coefficients as well as providing information on relaxation time distributions for the macromolecular components of complex systems. This volume is directed in part to the philosophy and current practice in dynamic light scattering. Single photon correlation techniques are introduced; a
discussion of noise on photon correlation functions is given and data analysis in dynamic light scattering to polymer structure analysis is presented; and a comprehensive introduction to diffusing wave spectroscopy is given. Theoretical developments relating dynamic light scattering to the
viscoelasticity of polymers in solution and in the bulk are described. A secondary aim of the work is to illustrate the widely varying fields in which the technique finds application. Chapters address multicomponent mixtures, polyelectrolytes, dense polymer systems, gels, rigid rods, micellar
systems and the application of dynamic light scattering to biological systems.

Effect of Ionizing Radiation on High Polymers

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Effect of Ionizing Radiation on High Polymers
T.S. Nikitina , and et al
Manufacturer: Gordon and Breach
ProductGroup: Book
Binding: Hardcover

The Effects of Radiation on High-Technology Polymers (Acs Symposium Series)

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The Effects of Radiation on High-Technology Polymers (Acs Symposium Series)

Manufacturer: An American Chemical Society Publication
ProductGroup: Book
Binding: Hardcover

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Book Description

This timely volume provides a review of fundamental radiation chemical processes and their relationship to several high technology industries. It demonstrates the significance of polymer radiation chemistry in industries such as electronics, aerospace, and optical recording. Six review
chapters and nine reports provide an in-depth look into fundamental radiation chemistry, recent advances in spectroscopic methods, applications of radiation-modified polymers in electronics and microlithography, and resistance of polymers to radiation. Research studies include polymer reaction to
radiation at the molecular level, energy dissipation pathways, microscopic properties of polymers, structure-property correlations, development of polymers for imaging at submicrometer resolution, and more.

Energy Transfer in Macromolecules (Press Monographs)

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Energy Transfer in Macromolecules (Press Monographs)
N. L. Vekshin
Manufacturer: SPIE-International Society for Optical Engine
ProductGroup: Book
Binding: Paperback

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ASIN: 0819420816

Free Atoms, Clusters, and Nanoscale Particles

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Free Atoms, Clusters, and Nanoscale Particles
Kenneth J. Klabunde
Manufacturer: Academic Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 0124107605

Book Description

This book presents coherent and systematic coverage of the broad and dynamic field of free atom and cluster atom chemistry. The text provides a comprehensive overview of the current literature and describes the mostimportant experimental techniques developed since 1980 including bimetallic clusters/catalysts, carbon clusters (fullerenes) and trapped single atoms. Metal atoms, clusters, and particles are covered in sequence with the Periodic Table.

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