Plant Functional Genomics (Methods in Molecular Biology, Vol. 236) (Methods in Molecular Biology)

Book Description

Erich Grotewold has assembled a team of leading plant scientists to describe in detail the most commonly used methods for investigating plant gene function in a wide variety of plants, during plant pathogen interactions, and even in algae. These readily reproducible protocols include computational, molecular, and genetic methodologies designed for both general and specific problems. Here the reader will learn about powerful computational and statistical tools to help predict gene function either on the basis of comparative genomics, or from the analysis of complex genome sequences. Numerous loss-of-function and gain-of-function techniques for discovering gene function are presented in step-by-step detail.

New Frontiers in Bryology: Physiology, Molecular Biology and Functional Genomics

Average customer rating:

New Frontiers in Bryology: Physiology, Molecular Biology and Functional Genomics

Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

General | Science | Subjects | Books

All Amazon Upgrade | Amazon Upgrade | Stores | Books

Professional & Technical | Amazon Upgrade | Stores | Books

Science | Amazon Upgrade | Stores | Books

All Titles | Qualifying Textbooks - Fall 2007 | Stores | Books

Professional | Qualifying Textbooks - Fall 2007 | Stores | Books

Science | Qualifying Textbooks - Fall 2007 | Stores | Books
ASIN: 1402019963

Book Description

The mosses (Bryophatea, Musci) are a diverse and widely distributed group of land plants. Mosses are attractive experimental plants because they exhibit the traditional attributes of good model systems (i.e. ease of growth and maintenance, fast generation time, and amenable genetics) with the added advantage of a haploid gametophyte that allowed developmental mutants to be recovered with relative ease. And, in one of the most exciting developments in Plant Biology, efficient homologous recombination occurs in the moss Physcomitrella patens. The ability to perform efficient homologous recombination (i.e. gene knock-outs) in P. patens is at present unique amongst all plants and represents an extremely powerful technique for the functional analysis of many plant genes. From evolution and development to the homologous recombination and the creation of novel molecular tools, mosses are being used to elucidate fundamental biological processes in plants. This book provides a synopsis of the outstanding basic research being conducted using mosses as a model multi-cellular eukaryote.

Average customer rating:

Extremely interesting

Plant Functional Genomics

Manufacturer: Food Products Press
ProductGroup: Book
Binding: Hardcover

Genetics | Evolution | Science | Subjects | Books

General | Science | Subjects | Books

All Titles | Qualifying Textbooks - Fall 2007 | Stores | Books
Similar Items:

Handbook of Formulas and Software for Plant Geneticists and Breeders

ASIN: 1560229985

Book Description

Discover cutting-edge knowledge for engineering a more productive and environment-friendly agriculture!

In Plant Functional Genomics, you'll find a cross-section of state-of-the-art research on the biological function of plant genes and how they work together in health and disease. World-leading scientists in the field present breakthrough techniques, discuss the results of projects aimed at dissecting particular plant functions, and provide an overview on the state of functional genomics for several plant and plant-related species. With figures, tables, and illustrations, this book will help scientists, researchers, and advanced students in botany find new ways of creating novel plant forms to better serve the needs of a rapidly expanding human population.

Plant Functional Genomics will increase your understanding of gene networks and systems rules, as well as gene expression during specific conditions or development or treatments. This important resource contains a wealth of data generated by various plant genome sequencing projects, including the newest results from experiments with Arabidopsis thaliana—the first plant to be completely sequenced. This book also contains innovative research on:

T-DNA mutagenesis

transcriptomics and metabolic profiling in plants

large-scale yeast two-hybrid analyses

the exceptional model system of Chlamydomonas genomics

functional genomics in rice, maize, and Physcomitrella

prospects for functional genomics in a new model grass

chloroplast and plant mitochondrial proteomics

plant transporters

so much more

Plant Functional Genomics will help speed up the identification and isolation of genes that might be of interest with respect to diverse biological questions. This valuable contribution to the field clarifies the challenges yet to be faced and the opportunities that could some day expand the frontiers of plant sciences.

Customer Reviews:

Extremely interesting.......2005-02-17

As the editor of this book explains in the preface, `functional genomics' is an extension of the term `genomics', with the latter used to designate the mapping and sequencing of genomes. Functional genomics, on the other hand, assumes the existence of a complete genome sequence, and then attempts to elucidate the functional properties of each gene. Motivated by the completion of the sequencing of the genome of Arabidopsis thaliana in 2000, the authors of the articles in this book discuss the more recent developments in plant functional genomics. Even though it is written for experts in the field, with some concentrated effort and a thorough perusal of the references, non-experts (such as this reviewer) who are curious about these developments should find the book worthwhile. Because of the interests of this reviewer, which lie predominantly in the biochemistry and biology of photosynthesis, and the genetic engineering of the chloroplast, the review will be restricted to the two articles that discuss these topics.

In the article `Chloroplast Proteomics' by Klaas J. van Wijk, the author believes that the study of the proteome of plastids is advantageous because of the reduced complexity of the problem, if compared to the proteomics of a total cell. The author illustrates this reductionist philosophy in the dividing up of the chloroplast proteome into six different "subproteomes" so as to make the identification and characterization of proteins more straightforward. These subproteomes are the inner and outer envelope, the soluble stroma, the tight and loose peripheral thylakoids, the soluble lumen, and the integral membrane. This division, the author believes, makes experimental determination of the proteins much more straightforward. He focuses his attention in the article on those experimental techniques that allow large sets of proteins to be predicted. Emphasis is placed on the way in which proteomics has contributed to the understanding of the biology of plastids. The author also takes great care in distinguishing large-scale transcript analysis from proteomic analysis, believing that both approaches are complementary, and gives references to the literature that support this viewpoint.

The role of statistical and mathematical techniques in proteomics, such as the use of hidden Markov models, is readily apparent in the article. Noting that the majority of the plastid proteome is encoded by the nuclear genome with the proteins being synthesized as precursor proteins with N-terminal `cleavable transit peptides' (cTPs), the author lists a few Websites for obtaining software, such as TargetP and Predotar, to identify cTPs and also lists other predictors and plastid proteomics databases cited in the chapter.

There are many very interesting facts reported in the chapter. One of these concerns the phylogenetic analysis of the proteome of Arabidopsis thaliana, which reveals that it has its origins in bacteria, but during the long process of evolution many other genes that encode for plastid proteins were acquired. Another concerns the use of hidden Markov models as predictors for alpha-helical transmembranes. Still another concerns the experimental techniques used for the identification and characterization of the plastid proteome, such as mass spectrometry, cyclotron resonance, and electrophoresis. Readers with knowledge of physics will certainly appreciate the discussion of these techniques.

In the article `Functional Characterization of the Photosynthetic Apparatus in Arabidopsis thaliana' by H. V. Scheller, C. Lunde, A. Haldrup, and P. E. Jensen, the authors emphasize that a substantial number of the genes of a plant, around ten percent, are involved in photosynthesis, and interestingly, have their origins in cyanobacteria. They are careful to point out both the advantages and disadvantages in using Arabidopsis to study photosynthesis, with the main advantage clearly being the availability of its complete genome. The primary focus of the article is on the function of proteins in the thylakoid membrane and the authors do not discuss stromal processes or transport processes in the chloroplast.

For the photosystem II (PSII) the authors give a list of the genes making up this system. They include in this list the location of the gene, i.e. whether it is in the nuclear or plastid genome, the protein for which it encodes, its molecular mass, its major function, and the accession number. Those readers interested in studying the effects on photosynthesis due to the switching off of these genes will find ample discussion in this article, with attention given to the nuclear-encoded subunits. The authors emphasize that not much is known about the chloroplast-encoded PSII proteins in Arabidopsis.

As an example of the effect of the knockout of genes, the authors discuss how the quantum yield of PSII electron transport and oxygen evolution can be decreased by the knockout in one of the psbO genes. They also discuss the destabilization of PSII dimers due to the reduction of the PsbW protein. This discussion is interesting in that no observable change in the phenotype was observed despite a fifty percent reduction in PSII activity.

Still another interesting discussion in this article regards the role of the Lhcb proteins in Arabidopsis, which are involved in light harvesting. For the Lhcb4 and Lhcb5 proteins, the authors describe an experiment in which these two proteins were down-regulated using an antisense approach. It is interesting to note that growth was not affected in the plants lacking Lhcb4 or Lhcb5, but that the PSII content was increased in the antisense plants. The authors conclude from this that the ratio between PSI and PSII is highly optimized in plants. A mutated plant with defects in one photosystem has a large capacity to compensate for the defects. For Lhcb2, the authors discuss an antisense experiment for one of the three lhcb2 genes that resulted in the largest number of gene products being simultaneously suppressed of any other study to date. Interestingly, the thylakoid structure was not affected by these manipulations, only small changes in the antenna size of PSII were observed, and the rate of photosynthesis was only reduced by about fifteen percent. Growth conditions were the same as the wild type in the laboratory, but fitness was considerably decreased under field conditions.

Average customer rating:

Functional Genomics

Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

Biochemistry | Biological Sciences | Science | Subjects | Books

Genetics | Evolution | Science | Subjects | Books

General | Science | Subjects | Books

All Amazon Upgrade | Amazon Upgrade | Stores | Books

Engineering | Amazon Upgrade | Stores | Books

Professional & Technical | Amazon Upgrade | Stores | Books

Science | Amazon Upgrade | Stores | Books

All Titles | Qualifying Textbooks - Fall 2007 | Stores | Books

Professional | Qualifying Textbooks - Fall 2007 | Stores | Books

Science | Qualifying Textbooks - Fall 2007 | Stores | Books
ASIN: 1402004567

Book Description

This volume provides an overview of the current state of plant genomics using a number of different approaches at a time when we celebrate the completion of the Arabidopsis genome sequence and begin the transition from structural to functional studies of this and other plant genomes. Topics covered include comparative genomics, computational approaches to gene identification and annotation and data management, high throughput methodologies for functional analysis at the levels of transcript, protein and metabolite, and methods for genome modification by both homologous and site-specific recombination. The book will provide a good introduction to some of the many aspects of genomics both for established plant biologists who wish to understand this rapidly developing area and for scientists early in their careers. It is also very suitable for a one-semester course in Plant Genomics at the upper-level undergraduate/graduate student level, where the individual chapters provide a framework that can be readily expanded by use of some of the many articles in the bibliographies.

Average customer rating:

Functional Plant Genomics

Manufacturer: Science Pub Inc
ProductGroup: Book
Binding: Hardcover

General | Science | Subjects | Books

All Titles | Qualifying Textbooks - Fall 2007 | Stores | Books
ASIN: 1578085063

Rice Functional Genomics: Challenges, Progress and Prospects

Average customer rating:

Rice Functional Genomics: Challenges, Progress and Prospects

Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

Genetics | Evolution | Science | Subjects | Books

General | Science | Subjects | Books

Food Science | Agricultural Sciences | Science | Subjects | Books

General | Reference | Science | Subjects | Books

All Titles | Qualifying Textbooks - Fall 2007 | Stores | Books
ASIN: 0387489037

Book Description

Rice has been chosen as the model cereal for functional genomics by the international scientific community not only because rice is a major food crop of the world but also because of its small genome (~430 Mb which is the smallest among cereal genomes); the ease with which it can be transformed; its well understood genetics with detailed genome physical maps and dense molecular markers; and the existence of great similarities in gene sequence, gene structure, gene order and gene function among all the cereals and grasses. Genes identified in rice as being important agronomically are also important in other cereals, and any understanding of rice genes is directly applicable to other cereals.

Rice Functional Genomics - Challenges, Progress and Prospects covers the whole spectrum of rice functional genomics. In addition to overviews of current status of genome sequencing and annotation, various tools and resources being developed worldwide such as expressed sequence tags (ESTs), full-length cDNAs, gene expression profiles (transcriptome, proteome and metabolome), chemical- and radiation-induced mutants, TILLING resources, insertional knockout mutants (T-DNA, transposon and retrotransposon) and activation tags have been described in various chapters. Exploitation of naturally occurring alleles, Oryza alignment maps (OMAP), gene targeting by homologous recombination and gene silencing by RNAi as well as the application of functional genomics tools for crop improvement and the power of comparative genomics are covered in separate chapters. Various bioinformatics tools and resources pertinent to rice functional genomics are also described and discussed. It is hoped that scientists involved in all aspects of rice research will find this book useful as it spans the divide between molecular biology and plant improvement.

Book Description

Chromatography is a major analytical technique that is used throughout research, development and manufacturing in the pharmaceutical, medical device and associated industries. To demonstrate fitness for purpose with the applicable regulations, the systems must be validated.

Validation of Chromatography Data Systems introduces the basics of computer validation. It looks in detail at the requirements throughout the life cycle of a CDS for any regulated laboratory, from its concept, through writing the user requirements specification to selecting the system, testing and operational release, including using electronic signatures. This logical and uniquely organised book provides the background to the regulatory requirements, interpretation of the regulations and documented evidence needed to support a claim that a system is validated. Development of the system, risk management, operation and finally system retirement and data migration are discussed. Case studies and practical examples are provided where appropriate.

Validation of Chromatography Data Systems is ideal for the chromatographer working in analytical laboratories in the regulated pharmaceutical, contract research, biotechnology and medical device industries seeking the practical guidance required for validating their chromatography data systems in order to meet regulatory requirements. It will also be welcomed by consultants or those in regulatory agencies.

Solid-Phase Extraction: Principles, Techniques, and Applications

Average customer rating:

Solid-Phase Extraction: Principles, Techniques, and Applications
Nigel J.K. Simpson
Manufacturer: CRC
ProductGroup: Book
Binding: Hardcover

Analytic | Chemistry | Science | Subjects | Books

General & Reference | Chemistry | Science | Subjects | Books

Industrial & Technical | Chemistry | Science | Subjects | Books

Chromatography | Chemistry | Science | Subjects | Books

General | Science | Subjects | Books

General & Reference | Technology | Science | Subjects | Books

Medical Technology | Allied Health Professions | Medicine | Subjects | Books

General | Arts & Photography | Subjects | Books

All Titles | Qualifying Textbooks - Fall 2007 | Stores | Books

Arts & Photography | Qualifying Textbooks - Fall 2007 | Stores | Books

Medicine | Qualifying Textbooks - Fall 2007 | Stores | Books

Professional | Qualifying Textbooks - Fall 2007 | Stores | Books

Science | Qualifying Textbooks - Fall 2007 | Stores | Books
Similar Items:

ASIN: 082470021X

Book Description

Demonstrating the relationship of the basic theory of solid-phase extraction (SPE) to chromatography, this comprehensive reference illustrates how SPE techniques significantly contribute to the preparation of samples for a wide variety of analytical techniques. It provides step-by-step details on the applications of SPE to environmental matrices, broad-spectrum drug screening, veterinary drug abuse, pharmaceutical drug development, biological samples, and high-throughput screening. Written by world-renowned experts in the field, the book contains helpful reference charts, tables of solvent properties, selectivities, molecular acid/base properties, and more.

1997 Annual Book of Astm Standards: General Test Methods, Nonmetal; Chromatography; Durability of Nonmetallic Materials; Forensic Sciences; Laboratory ... Book of a S T M Standards Volume 1402)

Average customer rating:

1997 Annual Book of Astm Standards: General Test Methods, Nonmetal; Chromatography; Durability of Nonmetallic Materials; Forensic Sciences; Laboratory ... Book of a S T M Standards Volume 1402)

Manufacturer: Astm Intl
ProductGroup: Book
Binding: Paperback

General | Engineering | Professional & Technical | Subjects | Books

General | Operating Systems | Computers & Internet | Subjects | Books
ASIN: 0803124554

Average customer rating:

Acol Hplc Software
Acol
Manufacturer: John Wiley & Sons
ProductGroup: Book
Binding: Hardcover

Analytic | Chemistry | Science | Subjects | Books

Crystallography | Chemistry | Science | Subjects | Books

General & Reference | Chemistry | Science | Subjects | Books

Chromatography | Chemistry | Science | Subjects | Books

General | Science | Subjects | Books

Computer Chromatography (Chromatographic methods)

Average customer rating:

Computer Chromatography (Chromatographic methods)
Kaiser
Manufacturer: Huthig Pub Ltd
ProductGroup: Book
Binding: Hardcover

Analytic | Chemistry | Science | Subjects | Books

A Computer-assisted Chromatography System (Chromatographic methods)

Average customer rating:

A Computer-assisted Chromatography System (Chromatographic methods)
Kiyokatsu Jinno
Manufacturer: Huethig Buch Verlag
ProductGroup: Book
Binding: Hardcover

Chromatography | Chemistry | Science | Subjects | Books
ASIN: 3778514970

Computer-Assisted Method Development for High-Performance Liquid Chromatography (Journal of Chromatography Library)

Average customer rating:

Computer-Assisted Method Development for High-Performance Liquid Chromatography (Journal of Chromatography Library)
Joseph L. Glajch
Manufacturer: Elsevier Science Ltd
ProductGroup: Book
Binding: Paperback

Analytic | Chemistry | Science | Subjects | Books

General & Reference | Chemistry | Science | Subjects | Books

Chromatography | Chemistry | Science | Subjects | Books

General | Computers & Internet | Subjects | Books
ASIN: 0444887482

Directory of Capillary Electrophoresis (Trac Supplement, No 1)

Average customer rating:

Directory of Capillary Electrophoresis (Trac Supplement, No 1)
D. Ed. Coleman
Manufacturer: Elsevier Science & Technology
ProductGroup: Book
Binding: Paperback

General & Reference | Chemistry | Science | Subjects | Books

Chromatography | Chemistry | Science | Subjects | Books

General | Science | Subjects | Books

Directories | Catalogs & Directories | Reference | Subjects | Books

Book Description

Paperback

Spectrum Extraction from Hyphenated Data (CD-ROM)

Average customer rating:

Spectrum Extraction from Hyphenated Data (CD-ROM)
E. J. Karjalainen , and U. P. Karjalainen
Manufacturer: Elsevier Science Ltd
ProductGroup: Book
Binding: CD-ROM

General & Reference | Chemistry | Science | Subjects | Books

Chromatography | Chemistry | Science | Subjects | Books

General | Digital Music | Computers & Internet | Subjects | Books
ASIN: 0444892257

Book Description

CD-ROM

Gas Chromatography's Mature Market Challenges Vendors.: An article from: Instrument Business Outlook

Average customer rating:

Gas Chromatography's Mature Market Challenges Vendors.: An article from: Instrument Business Outlook

Manufacturer: Strategic Directions International Inc. (SDI)
ProductGroup: Book
Binding: Digital

General | Business & Investing | Subjects | Books

Scheduling & E-mail | Microsoft | Computers & Internet | Subjects | Books

Book Description

This digital document is an article from Instrument Business Outlook, published by Strategic Directions International Inc. (SDI) on May 31, 1999. The length of the article is 1661 words. The page length shown above is based on a typical 300-word page. The article is delivered in HTML format and is available in your Amazon.com Digital Locker immediately after purchase. You can view it with any web browser.

Citation Details
Title: Gas Chromatography's Mature Market Challenges Vendors.
Publication: Instrument Business Outlook (Newsletter)
Date: May 31, 1999
Publisher: Strategic Directions International Inc. (SDI)
Volume: 8 Issue: 4 Page: NA

Distributed by Thomson Gale

Books:

Books Index

Books Home

Recommended Books