Atomic Force Microscopy: Biomedical Methods and Applications (Methods in Molecular Biology)

Book Description

Highly experienced physicians and biologists clearly explain the basic technical knowledge needed to use AFM and demonstrate its multifarious uses in biomedicine and the life sciences. The applications range widely from morphostructural analyses of cellular structures, to the investigation of subcellular structures, to functional investigations, and reveal a powerful new way of looking at biological samples. The methods clearly demonstrate the advantages of AFM over traditional life science microscopy, among them simultaneous very high magnification and resolution, minimal tissue and cell preparation, and the ability to obtain different views of the sample from a single data collection.

PCR Cloning Protocols: From Molecular Cloning to Genetic Engineering (Methods in Molecular Biology)

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PCR Cloning Protocols: From Molecular Cloning to Genetic Engineering (Methods in Molecular Biology)

Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 0896033430

Basic Protein and Peptide Protocols (Methods in Molecular Biology)

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Basic Protein and Peptide Protocols (Methods in Molecular Biology)

Manufacturer: Humana Press
ProductGroup: Book
Binding: Paperback

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ASIN: 0896032698

Book Description

Basic Protein and Peptide Protocols offers an excellent collection of reproducible, step-by-step laboratory methods covering three major areas: (1) the quantitation and characterization of proteins, (2) the electrophoretic and blotting procedures used in protein isolation and characterization, and (3) the analysis of protein and peptide structure. THOUSANDS of labs are already using Basic Protein and Peptide Protocols-you should be too!

Basic Cell Culture Protocols (Methods in Molecular Biology)

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Basic Cell Culture Protocols (Methods in Molecular Biology)

Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 1588292843

Book Description

In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side-population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis).

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A must for any student/post-doc in molecular biology

Basic Methods In Molecular Biology
Leonard G. Davis , Michael Kuehl , and James F. Battey
Manufacturer: McGraw-Hill Professional
ProductGroup: Book
Binding: Plastic Comb

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ASIN: 0838506429

Book Description

The new edition of this popular book emphasizes the decisions that need to be made to select one procedure over another.

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A must for any student/post-doc in molecular biology.......1999-05-17

The book is a cookbook collection of procedures and methods in molecular biology. It is written in a pragmatic and didactic fashion so that the understanding of the process is clear. There is a comprehensive list of reagents and solutions. It is well worth the buy!

Hormone Assays in Biological Fluids (Methods in Molecular Biology)

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Hormone Assays in Biological Fluids (Methods in Molecular Biology)

Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 1588290050

Book Description

Expert researchers who have developed and applied significant new assays describe in step-by-step detail a variety of methods for measuring a broad variety of hormones, related peptides, and synthetic steroids in various biological fluids. The hormones measured range from glucocorticoids in biological fluids, urinary steroids, aldosterone in blood, and plasma renin activity, to gut hormones in plasma, melatonin, prolactin, 6-sulfatoxymelatonin, and androgens in blood, saliva, and hair. The emphasis is on noncommercial assays so that investigators can set up novel methods suited to their special needs. Commercial assays are also described for comparative purposes. Tutorials on radioimmunoassay, gas chromatography-mass spectrometry, high-performance liquid chromatography, and PCR techniques help the reader to choose the best method for his or her purpose.

Flow Cytometry Data Analysis: Basic Concepts and Statistics

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Flow Cytometry Data Analysis: Basic Concepts and Statistics
James V. Watson
Manufacturer: Cambridge University Press
ProductGroup: Book
Binding: Paperback

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ASIN: 0521019702

Book Description

This book covers very basic number handling techniques, regression analysis, probability functions, statistical tests and methods of analyzing dynamic processes from flow cytometry data. These are developed for the analysis of not only individual DNA histograms to obtain the proportion of cells in the cell cycle phases, but also time courses of DNA histograms to yield cell cycle kinetic information; overlapping immunofluorescence distributions with confidence limits for the estimated proportions; enzyme kinetic and membrane transport parameters and a brief introduction to multivariate analysis is given. A distinction is made between data handling, for example gating and counting the numbers of cells within that gate, a process commonly regarded as data analysis but which, in reality, is data handling, and data analysis itself which is the means by which information is extracted.

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Basic Cell Culture Protocols (Methods in Molecular Biology)

Manufacturer: Humana Press
ProductGroup: Book
Binding: Paperback

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ASIN: 0896033848

Book Description

Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, 2nd ed. offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. Like the first edition, this book should be in every cell culture laboratory and be of use to all who use cell cultures in research.

Molecular Modeling: Basic Principles and Applications (Methods and Principles in Medicinal Chemistry, Vol 5)

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Accelrys advertisement

Molecular Modeling: Basic Principles and Applications (Methods and Principles in Medicinal Chemistry, Vol 5)
Hans-Dieter Holtje , Gerd Folkers , Thomas Beier , Wolfgang Sippl , and Didier Rognan
Manufacturer: Wiley-VCH Verlag GmbH
ProductGroup: Book
Binding: Hardcover

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ASIN: 3527293841

Book Description

Written by experienced experts in molecular modeling, this books describes the basics to the extent that is necessary if one wants to be able to reliably judge the results from molecular modeling calculations.
Its main objective is the description of the various pitfalls to be avoided. Without unnecessary overhead it leads the reader from simple calculations on small molecules to the modeling of proteins and other relevant biomolecules.
A textbook for beginners as well as an invaluable reference for all those dealing with molecular modeling in their daily work!

Customer Reviews:

Accelrys advertisement.......2007-01-17

This book describes what is essentially docking calculations in the context of the Accerlys software. The text is very qualitative and does not provide enough instruction to do any "molecular modeling". It seems to be more guidlines for various software packages sold by Accerlys.

Peptide Synthesis and Applications (Methods in Molecular Biology)

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Peptide Synthesis and Applications (Methods in Molecular Biology)

Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 1588293173

Book Description

Hands-on experts describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate their numerous applications. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included.

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Allen's commercial organic analysis;: A treatise on the properties, modes of assaying, and proximate analytical examination of the various organic chemicals ... adulterations, and products of decomposition
Alfred Henry Allen
Manufacturer: P. Blakiston's Son & Co
ProductGroup: Book
Binding: Unknown Binding

General & Reference | Chemistry | Professional Science | Professional & Technical | Subjects | Books
ASIN: B0006AFQYO

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Allen's commercial analysis;: A treatise on the properties, modes of assaying, and proximate analytical examination of the various organic chemicals and ... adulterations and products of decomposition
Alfred Henry Allen
Manufacturer: P. Blakiston's
ProductGroup: Book
Binding: Unknown Binding

Analytic | Chemistry | Science | Subjects | Books
General | Organic | Chemistry | Science | Subjects | Books
ASIN: B00088VG3E

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Commercial organic analysis: A treatise on the properties, proximate analytical examination, and modes of assaying the various organic chemicals and products ... adulterations, and products of decomposition
Alfred Henry Allen
Manufacturer: J. & A. Churchill
ProductGroup: Book
Binding: Unknown Binding

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ASIN: B00089GD0E

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